We narrowed to 88,170 results for: Mal

1. pEGFP_D2A_tdTomato

   Plasmid

   #184045

   Purpose

   Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

   Depositor

   Angela Pannier

   Article

   Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

   Inserts

   Enhanced Green Fluorescent Protein
   tdTomato
   Dual 2A Peptide Sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaCas9 TadDE

   Plasmid

   #193846

   Purpose

   Express TadDE (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE with SaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Myc:P4:gs:nls:ABI

   Plasmid

   #135985

   Purpose

   Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the ABI domain
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

   Plasmid

   #199348

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-TEV

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-E1.1-RFP

   Plasmid

   #22928

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   E1.1 binding site from Scardigli et al., 2003 with minimal CMV
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-mCherry-CyrCR1.miR16

   Plasmid

   #128747

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. OVA-miSFIT-17-T

   Plasmid

   #124677

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ppBAalbNixE1E2 OpIE2-GFP

   Plasmid

   #173505

   Purpose

   transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Inserts

   Nix masculinisation gene
   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Nix isoforms with exons 1, 2 separated by intron 1

   Promoter

   OpIE2 and own

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT (REP10)

   Plasmid

   #21368

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eNme2-C TadDE

    Plasmid

    #193842

    Purpose

    Express TadDE (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadDE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IZ-ANXA4-mRuby3

    Plasmid

    #184890

    Purpose

    Stable expression of ANXA4-mRuby3 (Annexin A4-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA4 (ANXA4 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-CMP-PE-V1

    Plasmid

    #202769

    Purpose

    Expresses CMP-PE-V1 in mammalian cells for prime editing

    Depositor

    Kyoungmi Kim

    Article

    Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

    Insert

    CMP-PE-V1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PGK+ episome

    Plasmid

    #170041

    Purpose

    PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SMVP-Cas9N-U6-gRNA TdR

    Plasmid

    #80940

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV+ episome

    Plasmid

    #169742

    Purpose

    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_SPIB_P2A_Hygro_Barcode

    Plasmid

    #120483

    Purpose

    Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SPIB (SPIB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT (AF20)

    Plasmid

    #21359

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available Since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-FKBP-Rab5-Blasticidin

    Plasmid

    #120715

    Purpose

    Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Rab5a
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-215

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-FLAG-CaMKIID-K43M

    Plasmid

    #186830

    Purpose

    Expresses FLAG-CaMKIID-K43M in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2d (Camk2d Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 43 to Methionine

    Promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX-FKBP-Rab11-Blasticidin

    Plasmid

    #120717

    Purpose

    Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Rab11a
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-216

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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