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pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC1

Plasmid

#191856

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AiP14460: pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BarcodeR-BGHpA (Alias: CN4460)

Plasmid

#236599

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0452h_3xC2 is an enhancer active in striatal D2-MSNs and cortical L5_ET neurons

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14454: pAAV-AiE0783m-minBG-SYFP2-WPRE3-BarcodeL-BGHpA (Alias: CN4454)

Plasmid

#236593

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0783m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14453: pAAV-AiE0441h-minBG-SYFP2-WPRE3-BarcodeK-BGHpA (Alias: CN4453)

Plasmid

#236592

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0441h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14449: pAAV-AiE0367h_3xC2-minRho-SYFP2-WPRE3-BarcodeG-BGHpA (Alias: CN4449)

Plasmid

#236588

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0367h_3xC2 is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14461: pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BarcodeS-BGHpA (Alias: CN4461)

Plasmid

#236600

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0779m_3xC2 is an enhancer active in striatal D1-MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14458: pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BarcodeP-BGHpA (Alias: CN4458)

Plasmid

#236597

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0441h_3xC2 is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14456: pAAV-AiE0603m-minBG-SYFP2-WPRE3-BarcodeN-BGHpA (Alias: CN4456)

Plasmid

#236595

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0603m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14459: pAAV-AiE0785m-minBG-SYFP2-WPRE3-BarcodeQ-BGHpA (Alias: CN4459)

Plasmid

#236598

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0785m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14457: pAAV-AiE0784m-minBG-SYFP2-WPRE3-BarcodeO-BGHpA (Alias: CN4457)

Plasmid

#236596

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0784m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14448: pAAV-3xSP10ins-AiE0367h-minRho-SYFP2-WPRE3-BarcodeA-BGHpA (Alias: CN4448)

Plasmid

#236587

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0367h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14455: pAAV-AiE0526h-minBG-SYFP2-WPRE3-BarcodeM-BGHpA (Alias: CN4455)

Plasmid

#236594

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0526h is an enhancer active in striatal MSNs and cortical Sst neurons

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14451: pAAV-AiE0447h-minBG-SYFP2-WPRE3-BarcodeI-BGHpA (Alias: CN4451)

Plasmid

#236590

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0447h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14450: pAAV-AiE0450h-minBG-SYFP2-WPRE3-BarcodeH-BGHpA (Alias: CN4450)

Plasmid

#236589

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0450h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14452: pAAV-3xSP10ins-AiE0351h-minBG-SYFP2-WPRE3-BarcodeJ-BGHpA (Alias: CN4452)

Plasmid

#236591

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0351h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge