We narrowed to 16,140 results for: IGH@

1. H6Gb1-CREB(1-280) C90A, T172C, T259C

   Plasmid

   #220234

   Purpose

   bacterial expression of CREB1 domains

   Depositor

   Peter Wright

   Article

   Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   C90A, T172C, T259C

   Promoter

   T7

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCBE3-HF1

   Plasmid

   #218156

   Purpose

   This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCBE3-HF1-Hypa

   Plasmid

   #218158

   Purpose

   This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCBE3-Hypa

   Plasmid

   #218157

   Purpose

   This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N692A…

   Promoter

   rpsL promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. kanMX6-ins1

   Plasmid

   #195038

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-hSOX2AV

   Plasmid

   #193352

   Purpose

   Retroviral vector expressing human SOX2A61V point mutant

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2AV (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   SOX2A61V is a highly cooperative point mutant of …

   Promoter

   5'LTR

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-SAPAP1/2 [N238/31R]

   Plasmid

   #182110

   Purpose

   Mammalian Expression Plasmid of anti-SAPAP1/2 (). Derived from hybridoma N238/31.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-SAPAP1/2 () recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CCDC-bio-His

   Plasmid

   #53331

   Purpose

   Expresses full-length Coiled-coil domain-containing protein 109A precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CCDC (MCU Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. G6f-bio-His

    Plasmid

    #53342

    Purpose

    Expresses full-length Lymphocyte antigen 6 complex locus protein G6f precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    G6f (LY6G6F Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. DCBL-bio-His

    Plasmid

    #51867

    Purpose

    Expresses full-length Discoidin, CUB and LCCL domain-containing protein 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    DCBL (DCBLD2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. AP1muA-TurboID-polyA-G418 HR

    Plasmid

    #227734

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-GST [N100/13R ]

    Plasmid

    #188214

    Purpose

    Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. plx304-PHB-V5

    Plasmid

    #122230

    Purpose

    Expresses human PHB attached to a V5 tag

    Depositor

    Alex Kentsis

    Article

    Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

    Insert

    Prohibitin (PHB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYLCRISPR/Cas9P35S-H

    Plasmid

    #66189

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. ITGB1

    Plasmid

    #51920

    Purpose

    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Stop Codon at amino acid 728, before CD4, bio and…

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYLCRISPR/Cas9P35S-N

    Plasmid

    #66191

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYLCRISPR/Cas9P35S-B

    Plasmid

    #66190

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR-BLINK2

    Plasmid

    #117075

    Purpose

    BLINK2 gene in pDONR vector for Gateway cloning system

    Depositor

    Anna Moroni

    Article

    Alberio et al Nat Methods. 2018 Nov;15(11):969-976. do

    Insert

    BLINK2
    

    Use

    Gateway cloning system

    Tags

    Expression

    Mutation

    glutamine 112 changed into aspartate (original LO…

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

    Plasmid

    #171619

    Purpose

    AAV floxed Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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