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We narrowed to 3,796 results for: mak

Showing: 3121 - 3140 of 3796 results
  1. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    Insert
    Mb 64Y68F
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pb-CAGGS-dCas9-blasto

    Plasmid
    #218555
    Purpose
    Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance
    Depositor
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid
    #32522
    Depositor
    Insert
    dominant negative Rheb (D60K) (Rheb Rat)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    D60K to make dominant negative
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
  6. pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

    Plasmid
    #32521
    Depositor
    Insert
    Dominant negative Rheb (D60I) (Rheb Rat)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    D60I to make dominant negative
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
  7. PB-Puro-U6-gRNA-pA plasmid

    Plasmid
    #247671
    Purpose
    spCas9 gRNA is driven by human U6 promoter, with the gRNA cassette between PuroR and pA, making it detectable in polyA-based RNA-seq.
    Depositor
    Insert
    Puromycin resistant gene
    Expression
    Mammalian
    Promoter
    TK promoter
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pBrg1-GGSG_AID_3xF_PP Target2

    Plasmid
    #235523
    Purpose
    Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed PgkPuro
    Depositor
    Insert
    Smarca4 (Smarca4 Mouse)
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Mutation
    None
    Promoter
    PGK
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pGEMHE-TNKS-2-M1054V

    Plasmid
    #138538
    Purpose
    For making TNKS-2-M1054V RNA
    Depositor
    Insert
    Tankyrase 2 (TNKS2 Human)
    Use
    In vitro transcription
    Tags
    FLAG
    Mutation
    Changed methionine 1054 to valine
    Promoter
    T7
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 3121 - 3140 of 3796 results