We narrowed to 9,402 results for: pho;

1. pLEX301-ARF6-T157N-TagRFP

   Plasmid

   #162030

   Purpose

   Fast cycling ARF mutant

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Lentiviral

   Mutation

   T151N

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX CblB-SH2

   Plasmid

   #46413

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   CblB (CBLB Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 42-359

   Promoter

   Tac

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

   Plasmid

   #69117

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   LL (566-567aa) mutated to AA. Silent mutations i…

   Promoter

   CMV

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-FLAG-IRES-Blasticidin-hOCT4 WT

   Plasmid

   #198764

   Purpose

   Mammalian expression of hOCT4

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

   Insert

   hOCT4 WT (POU5F1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-Flt3-Y591F/Y919F

   Plasmid

   #74498

   Purpose

   Retroviral expression of FLT3 Y591F/Y919F

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   unknown

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGEX 4T-1 GST-Ago2 S387A

   Plasmid

   #60656

   Purpose

   Expresses GST-Ago2 S387A mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S387A

   Promoter

   tac

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Btk-SH2

   Plasmid

   #46412

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Btk (BTK Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 271-385

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-AR-R405A

   Plasmid

   #126052

   Purpose

   Expresses human androgen receptor (R405A) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

   Insert

   AR (AR Human)
   

   Expression

   Mammalian

   Mutation

   R405A

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE GFP-Ago2 S387E

   Plasmid

   #60655

   Purpose

   GFP-Ago2 S387E mutant lentiviral vector

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   S387E

   Promoter

   CMV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid

    #82031

    Purpose

    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Non phosphorylatable mutations on 3 of the main A…

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag‐mTim3 (del.Cyto)

    Plasmid

    #49209

    Purpose

    expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (del.Cyto) (Havcr2 Mouse)
    

    Tags

    CD8 signal sequence and FLAG

    Expression

    Mammalian

    Mutation

    cytosolic domain deleted, no Tim3 signal sequence…

    Promoter

    Ef1a

    Available Since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

    Plasmid

    #99261

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    Y705F (contains a silent mutation: nucleotide 271…

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gal4-VP16-HSF1 WT

    Plasmid

    #71726

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)
    

    Expression

    Mammalian

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Blnk-SH2

    Plasmid

    #46408

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Blnk (BLNK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 333-456

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1+_LN-HA-AGO2-5xA

    Plasmid

    #92010

    Purpose

    Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. rd-his

    Plasmid

    #112911

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Tags

    6-his tag, Glutathione-S-Transferase, and preciss…

    Expression

    Bacterial

    Mutation

    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…

    Promoter

    T7

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5 Flag p38 alpha(agf)

    Plasmid

    #20787

    Depositor

    Roger Davis

    Article

    Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

    Insert

    pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced Thr180 and Tyr182 with Ala and Phe, resp…

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pGEX Grap2-SH2

    Plasmid

    #46435

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grap2 (GRAP2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 52-159

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Fgr-SH2

    Plasmid

    #46427

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Fgr (FGR Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 137-245

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-AR-R405S

    Plasmid

    #126051

    Purpose

    Expresses human androgen receptor (R405S) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Expression

    Mammalian

    Mutation

    R405S

    Promoter

    CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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