Skip to main content
Addgene
Showing: 6281 - 6320 of 13641 results

  2. pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

    Plasmid
    #32521
    Depositor
    Mustafa Sahin
    Insert
    Dominant negative Rheb (D60I) (Rheb Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D60I to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  3. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Daniel Durocher
    Article
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Insert
    RNF168 (RNF168 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133983-plasmid-map-sequence-id-261913

    Enlarge

  4. pLV-Enh eGFP Reporter-muHRNR-X50

    Plasmid
    #59451
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of mouse HMR.
    Depositor
    Benjamin Yu
    Insert
    Hrnr (Hrnr Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

    Enlarge

  6. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175784
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Use
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175784-plasmid-map-sequence-id-345284

    Enlarge

  7. pLV-Enh eGFP Reporter-muKC2-IGR13

    Plasmid
    #59282
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    17kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid
    #156414
    Purpose
    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4B-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    mutant KRAS4B-G12V
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156414-plasmid-map-sequence-id-308707

    Enlarge

  12. pLV-Enh eGFP Reporter-muEDC-Int3

    Plasmid
    #59288
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.
    Depositor
    Benjamin Yu
    Insert
    mFlg int-Intron 1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pLV-Enh eGFP Reporter-muKC2-X39

    Plasmid
    #59263
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt83-ex5-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. integrin alpha9 / alpha5 EGFP pBabe puro

    Plasmid
    #13608
    Depositor
    Dean Sheppard
    Insert
    integrin alpha 9 / alpha 5 (ITGA9 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Integrin alpha 9 chimera containing the cytoplasm…
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only
    13608-plasmid-map-sequence-id-254609

    Enlarge

  18. pLV-Enh eGFP Reporter-muKC2-X33

    Plasmid
    #59257
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt81-ex4-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pLV-Enh eGFP Reporter-muKC2-X14

    Plasmid
    #59238
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Krt8-ex5-8
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pLV-Enh eGFP Reporter-muKC2-IGR12

    Plasmid
    #59281
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    1.5kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pWPT-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49232
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49232-plasmid-map-sequence-id-73653

    Enlarge

  22. pCru5-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49221
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49221-plasmid-map-sequence-id-73631

    Enlarge

  23. pCru5-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49223
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49223-plasmid-map-sequence-id-73635

    Enlarge

  27. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

    Plasmid
    #238283
    Purpose
    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG
    Depositor
    Denes Hnisz
    Insert
    mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238283-plasmid-map-sequence-id-474433

    Enlarge

  30. AAV-hSyn-PiGM-Ii (173, 535/81, eGFP)

    Plasmid
    #221611
    Purpose
    AAV encoding the 173 amino acid version PiGM-Ii with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Ii (173, eGFP)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS10(truncation after amino acid 173)
    Promoter
    Human Synapsin
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    221611-plasmid-map-sequence-id-461235

    Enlarge

  35. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid
    #223123
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223123-plasmid-map-sequence-id-447704

    Enlarge

  36. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid
    #223125
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223125-plasmid-map-sequence-id-447703

    Enlarge

  37. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid
    #223102
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223102-plasmid-map-sequence-id-447669

    Enlarge

  38. pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

    Plasmid
    #223103
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223103-plasmid-map-sequence-id-447689

    Enlarge

  39. pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

    Plasmid
    #223104
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223104-plasmid-map-sequence-id-447670

    Enlarge

  40. pCMV-T7-SpCas9(MRKSKC)-P2A-EGFP (RAS2840)

    Plasmid
    #223105
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRKSKC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKSKC)=D1135M, S1136R, G1218K, E1219S, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223105-plasmid-map-sequence-id-447671

    Enlarge

Showing: 6281 - 6320 of 13641 results