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p3039 pVL E6-AP

Plasmid

#8646

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Insect

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
Cas-Acr (D14A/G38A)

Plasmid

#128118

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (D14A/G38A)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBS cCollagen XII (CT#1051)

Plasmid

#13916

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Collagen XII (COL12A1 Chicken)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

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p3004 pGEM E6-AP 76kD

Plasmid

#10857

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Mutation

76kD: amino acids 213-865

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pBS cFgfr2 (CT#221)

Plasmid

#14003

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Fgfr2 (FGFR2 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
p3012 pGEM E6-AP C-A

Plasmid

#8645

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Mutation

active site cyteine codon mutated to encode alani…

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pBS cPea3 (CT#1024)

Plasmid

#13906

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (ETV4 Chicken)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
p3045 pVL E6-AP C-A

Plasmid

#8647

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Insect

Mutation

Active site Cys mutated to encode Ala

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pLENTICRISPR V2 hygro sgmPOLE_2

Plasmid

#241443

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_2 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 hygro sgmPOLE_1

Plasmid

#241442

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_1 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pJV457

Plasmid

#180080

Purpose

M.BbrUI expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUI

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV461

Plasmid

#180081

Purpose

M.BbrUIII expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUIII

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE

Plasmid

#233264

Purpose

Backbone plasmid used to clone lncRNA inserts in preparation for RMCE

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Type

Empty backbone

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3GFP

Plasmid

#233008

Purpose

Galactose iduced expression of Gcn4 E+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IHA

Plasmid

#232993

Purpose

Galactose iduced expression of Gcn4 F124IHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_FL Gcn4

Plasmid

#232955

Purpose

Galactose iduced expression of full-length Gcn4 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+

Plasmid

#232982

Purpose

Galactose iduced expression of Gcn4 Aro2+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+

Plasmid

#232983

Purpose

Galactose iduced expression of Gcn4 Aro3+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AHA

Plasmid

#232992

Purpose

Galactose iduced expression of Gcn4 W120AHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDHA

Plasmid

#232987

Purpose

Galactose iduced expression of Gcn4 DBDAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IGFP

Plasmid

#233005

Purpose

Galactose iduced expression of Gcn4 F124IGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 L123A

Plasmid

#232969

Purpose

Galactose iduced expression of Gcn4 L123A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap2

Plasmid

#232965

Purpose

Galactose iduced expression of Gcn4 k0.19 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.19

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 W120AGFP

Plasmid

#233004

Purpose

Galactose iduced expression of Gcn4 120AGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 K5

Plasmid

#232966

Purpose

Galactose iduced expression of Gcn4 K+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3

Plasmid

#232960

Purpose

Galactose iduced expression of Gcn4 k0.47 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge