We narrowed to 5,876 results for: nls

1. SGEP-sh-Hs-PHGDH-1968

   Plasmid

   #188673

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SGEP-sh-Hs-PHGDH-1957

   Plasmid

   #188674

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ipUSEPR-sg-Hs-PHGDH-2

   Plasmid

   #188676

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SGEP-sh-Hs-PHGDH-1967

   Plasmid

   #188672

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ipUSEPR-sg-Hs-PHGDH-5

   Plasmid

   #188679

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ipUSEPR-sg-Hs-PHGDH-6

   Plasmid

   #188680

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ipUSEPR-sg-Hs-PHGDH-4

   Plasmid

   #188678

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ipUSEPR-sg-Hs-PHGDH-3

   Plasmid

   #188677

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTagRFP-T2-PCNA-19

   Plasmid

   #58043

   Purpose

   Localization: Nucleus, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL1180-HR-PUbECFP

    Plasmid

    #47917

    Purpose

    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    ECFP
    

    Use

    Tags

    NLS

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti polyubiquitin (PUb)

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TC9nickpAB

    Plasmid

    #71311

    Purpose

    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9 nickase
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    D10A nickase

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid

    #188682

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid

    #188681

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid

    #188683

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-SasgRNA scaffold-CMV-nSaCas9

    Plasmid

    #171695

    Purpose

    Expresses nSaCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-nSaCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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