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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,500 results for: bon;

Showing: 7951 - 8000 of 17500 results

  1. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available Since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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  2. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  4. PP-RNR

    Plasmid
    #25135
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PY03671:C21955 (PY03671 Plasmodium yoelii)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pDC10

    Plasmid
    #75077
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase (purD Escherichia coli)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    adds unique SacII site at Thr95-Ala96 by a silent…
    Promoter
    T7
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    75077-plasmid-map-sequence-id-142310

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  10. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  14. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220981-plasmid-map-sequence-id-437540

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  21. pMulti_S_ccdB

    Plasmid
    #223853
    Purpose
    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)
    Depositor
    Stephan Gruber
    Article
    Taschner et al bioRxiv 2024.06.17.599261
    Type
    Empty backbone
    Use
    Golden-gate acceptor
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    223853-plasmid-map-sequence-id-454892

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  30. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99248-plasmid-map-sequence-id-191832

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  31. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid
    #175571
    Purpose
    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175571-plasmid-map-sequence-id-344994

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  35. pRDB_345

    Plasmid
    #231113
    Purpose
    ABE activity-based selection cloning vector
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231113-plasmid-map-sequence-id-458424

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  37. pRDB_347

    Plasmid
    #231114
    Purpose
    CBE activity-based selection cloning vector
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231114-plasmid-map-sequence-id-458384

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  47. pLV-DBNF-mCherry

    Plasmid
    #187195
    Purpose
    To express the mouse BDNF tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF
    Use
    Lentiviral
    Tags
    fluorescent protein mCherry
    Expression
    Mammalian
    Mutation
    The codons have been optimized for better express…
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187195-plasmid-map-sequence-id-374465

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Showing: 7951 - 8000 of 17500 results