We narrowed to 5,331 results for: pcdna3.1

1. Cenp-B INCENP mCherry

   Plasmid

   #45235

   Purpose

   Target INCENP to centromeres via full length CENP-B

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B, INCENP-Δcen, mCherry
   

   Tags

   full length CENP-B and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAL190

   Plasmid

   #22279

   Purpose

   Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4xHRE_minSV40-sfGFP-CMV_dsRed

   Plasmid

   #117400

   Purpose

   sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

   Depositor

   Yvonne Chen

   Article

   Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

   Insert

   SuperFolder GFP
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-ELYS-emerin-EGFP-polyA

   Plasmid

   #59745

   Purpose

   Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   ELYS-emerin-EGFP
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 (+) Tacδ

   Plasmid

   #81177

   Purpose

   Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

   Depositor

   Chinten James Lim

   Article

   Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

   Insert

   Tacδ
   

   Tags

   KLGFFKR

   Expression

   Mammalian

   Mutation

   CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cenp-B-targeted Aurora B FRET sensor

   Plasmid

   #45232

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Expression

   Mammalian

   Promoter

   t7

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-RNF126 C231A

   Plasmid

   #138648

   Purpose

   Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

   Depositor

   Jane McGlade

   Article

   Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

   Insert

   RNF126 (RNF126 Mouse)
   

   Tags

   HA-HA

   Expression

   Mammalian

   Mutation

   C231A

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-YOD1 C160S

   Plasmid

   #49785

   Purpose

   Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

   Insert

   YOD1 (YOD1 Human)
   

   Tags

   Flag-tag

   Mutation

   C160S

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-NBid-mMaple-CBid

   Plasmid

   #164049

   Purpose

   Optogenetic manipulation of cell apoptosis negative control

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NBid-mMaple-CBid
   

   Tags

   kozak sequence

   Expression

   Mammalian

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNIR-Fb2E7

    Plasmid

    #184681

    Purpose

    NIR-Fb to HIV gp41

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb2E7
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRPML1-MYC

    Plasmid

    #18824

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOVC1_EcSSPB-nano_X

    Plasmid

    #131212

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HIPK1-myc-his

    Plasmid

    #1850

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    HIPK1 (Hipk1 Mouse)
    

    Tags

    his and myc

    Expression

    Mammalian

    Available Since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

15. OSCA1.2-PP-GFP

    Plasmid

    #135390

    Purpose

    Expresses OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Tags

    EGFP tag connected to the gene via a precision pr…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAL189

    Plasmid

    #22278

    Purpose

    Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. VAChT-SNAP

    Plasmid

    #105318

    Purpose

    VAChT tagged with SNAP

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAChT (Slc18a3 Rat)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRAPa-3xFLAG-pCDNA

    Plasmid

    #201963

    Purpose

    Mammalian expression of Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Available Since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRPML3-CFP

    Plasmid

    #18834

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML3 (Mcoln3 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q165R, F210Y

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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