We narrowed to 16,111 results for: form

1. pHR-SUMO-A'-l4-G'-IRES-EmGFP

   Plasmid

   #209127

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'-IRES-EmGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-SUMO-A'-l4-G-IRES-EmGFP

   Plasmid

   #209126

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G-IRES-EmGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA_B2AR-q-pep

   Plasmid

   #47437

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

   Plasmid

   #195049

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Expression

   Bacterial

   Promoter

   pPGI

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX-IY LC3C

   Plasmid

   #89292

   Purpose

   For expression of the mammalian Atg8 protein LC3C without a tag

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   LC3C (MAP1LC3C Human)
   

   Use

   Retroviral

   Tags

   IRES-YFP

   Expression

   Mammalian

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA

   Plasmid

   #108805

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108806

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

   Plasmid

   #121831

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/LSD1 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hEBI3 pEF6-V5 (MJS_002)

   Plasmid

   #72490

   Purpose

   Expression of human EBI3 in mammalian cells (V5 tag)

   Depositor

   Matthew Sweet

   Article

   Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

   Insert

   EBI3 (EBI3 Human)
   

   Tags

   His/V5

   Expression

   Mammalian

   Promoter

   EF-1 alpha

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

    Plasmid

    #108795

    Purpose

    Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa571
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

    Plasmid

    #108796

    Purpose

    Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa572 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FKBP and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Erk2

    Plasmid

    #40777

    Purpose

    Expression of rat Erk2 in mammalian cells

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

    Insert

    Erk2 (Mapk1 Rattus norvegicus)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP-C1-mApg5(K130R)

    Plasmid

    #22959

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    130 Lysine to Arginine

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

14. FN-Ets2

    Plasmid

    #44603

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 (Ets2 Mouse)
    

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Syn-FLEX-Mac-GFP

    Plasmid

    #58852

    Purpose

    AAV-mediated expression of Mac-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Insert

    Mac-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV5-Flag-EPLIN delta N-term

    Plasmid

    #40932

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-220

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCMV 3xFLAG-GABARAP G116

    Plasmid

    #123099

    Purpose

    Expresses 3xFLAG-GABARAP G116 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-BG505.DS-SOSIP.4mut-gp160

    Plasmid

    #123277

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variant

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBV-Luc/Del-6

    Plasmid

    #14969

    Depositor

    Joan Massague

    Article

    Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    c-myc promoter (-109/+334) (MYC Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -109/+334 (relative to the P2 transcription initi…

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid

    #192443

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p3E-CDC42-DN

    Plasmid

    #109584

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pCMV5-Flag-EPLIN alpha delta C-term

    Plasmid

    #40930

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 296-600

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pCEP4-BG505.DS-SOSIP.6mut-gp160

    Plasmid

    #123278

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDB.His.MBP.3C NLRP1 CARD WT

    Plasmid

    #164008

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LN-entry+NLS

    Plasmid

    #128011

    Purpose

    Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    LN-3xFlag-SV40NLS
    

    Use

    Lamdan entry vector with nls to generate tetherin…

    Tags

    LN-3xFlag-SV40NLS

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

    Plasmid

    #184334

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

    Plasmid

    #214877

    Purpose

    Lentiviral vector encoding RfxCas13d targeting LPT guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crLPT-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223 LC3B no-stop

    Plasmid

    #123205

    Purpose

    Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223 LC3A no-stop

    Plasmid

    #123204

    Purpose

    Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. eGFP-KCC2

    Plasmid

    #104075

    Purpose

    expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

    Depositor

    Igor Medyna

    Article

    Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    CMV

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSK+ mBMPR-1B DN

    Plasmid

    #49530

    Purpose

    cloning vector for dominant negative mouse BMP receptor 1B

    Depositor

    Peter ten Dijke, Lee Niswander

    Article

    Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

    Insert

    BMPR-1B DN (Bmpr1b Mouse)
    

    Expression

    Bacterial

    Mutation

    dominant negative mutation (K231R); also L2V

    Promoter

    T3

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pEGFP-PTRF

    Plasmid

    #68401

    Purpose

    Expression of fluorescently tagged PTRF in mammalian cells

    Depositor

    Rob Parton

    Article

    Hill et al Cell. 2008 Jan 11;132(1):113-24. doi: 10

    Insert

    PTRF (Cavin1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

34. TOPO UBC

    Plasmid

    #68456

    Purpose

    TOPO construct expressing UBC for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Ubiquitin C Promoter (UBC Human)
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. B1 Integrin

    Plasmid

    #186137

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Tags

    MBP and untranslated His6 after stop codon

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #108769

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Unc5c.a-Fc-His

    Plasmid

    #72180

    Purpose

    Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.a (Unc5c Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Ncam1_1.18.1-AP-His

    Plasmid

    #71962

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.18.1 (Ncam1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMXS-IRES-Blast SHMT2res

    Plasmid

    #106301

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Silent mutations destroy sgRNA targeting sites

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Ncam1_0.0.0-AP-His

    Plasmid

    #71960

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_0.0.0 (Ncam1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV 3xFLAG-LC3A G120A

    Plasmid

    #123090

    Purpose

    Expresses 3xFLAG-LC3A G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pMRX-IP/Venus-mATG5

    Plasmid

    #58739

    Purpose

    Expresses Venus-mATG5 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pENTR221-CCL5.Pro

    Plasmid

    #79492

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    CCL5 Promoter (CCL5 Synthetic, Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    CCL5 Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCMV 3xFLAG-GABARAPL1 G116A

    Plasmid

    #123101

    Purpose

    Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. HP-Cas9hGem-Ade2-LEU2-LINEAR

    Plasmid

    #174839

    Purpose

    pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Yeast

    Promoter

    TDH3p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. p3E-CDC42

    Plasmid

    #109583

    Purpose

    Multisite gateway vector for 3' tagging with CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. 7432 Bicistronic_GFP_ires_puro

    Plasmid

    #64336

    Purpose

    This a retroviral expression plasmid expressing GFP along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    GFP

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pDONR223 GABARAPL2 G116A

    Plasmid

    #123087

    Purpose

    Gateway entry clone encoding human GABARAPL2 G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 116 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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