We narrowed to 11,674 results for: NSI

1. MCP-EGFP-PiggyBac

   Plasmid

   #190003

   Purpose

   Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

   Depositor

   Tineke Lenstra

   Article

   Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

   Insert

   MCP-EGFP
   

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSGAb-spe

   Plasmid

   #122000

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-EF1a-TetOn3G

   Plasmid

   #104543

   Purpose

   PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   Tet-On 3G
   

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM5057

   Plasmid

   #120085

   Purpose

   Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

   Depositor

   Susan Golden

   Article

   Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

   Insert

   RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
   

   Use

   Other

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7-LacO-LgBiT

   Plasmid

   #216779

   Purpose

   Expresses LgBiT in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   LgBiT
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-R-Rac1

   Plasmid

   #180576

   Purpose

   ddRFP sensor of Rac1

   Depositor

   Won Do Heo

   Article

   Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

   Insert

   ddRFP sensor of Rac1
   

   Expression

   Mammalian

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-G-Rac1

   Plasmid

   #180575

   Purpose

   ddGFP sensor of Rac1

   Depositor

   Won Do Heo

   Article

   Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

   Insert

   ddGFP sensor of Rac1
   

   Expression

   Mammalian

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT05

   Plasmid

   #223377

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT01

   Plasmid

   #223373

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTalpha1-Dre

    Plasmid

    #133925

    Purpose

    Neuron-specific expression of Dre

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    HA-Dre
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pP4-C

    Plasmid

    #225097

    Purpose

    gfpUV reporter vector with a high performance adenine-dependent riboswitch

    Depositor

    Robert Batey

    Article

    Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

    Insert

    synthetic riboswitch
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVEX2.3-Theo-3c-EGFP

    Plasmid

    #217526

    Purpose

    Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

    Depositor

    Keisuke Fukunaga

    Article

    Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

    Insert

    Theo-3c riboswitch-regulated EGFP
    

    Use

    In vitro transcription-translation

    Promoter

    T7

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pN3-mTagBFP-pHluorin-CAAX

    Plasmid

    #178345

    Purpose

    Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    mTagBFP-pHluorin-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG1243 NES-ECFP-FIREmate

    Plasmid

    #205169

    Purpose

    Expresses NES-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NES-ECFP-FIREmate
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG1188 mCherry-cagedFIREtag

    Plasmid

    #205177

    Purpose

    Expresses mCherry-cagedFIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag-DEVDG-MoA
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMH48

    Plasmid

    #131861

    Purpose

    Produces holo-Cph1* in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

    Inserts

    Cph1(1-514,Y263F)-RGD-His6-Cys
    HO1
    PcyA
    

    Expression

    Bacterial

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG1171 FIREmate-ECFP-Giantin

    Plasmid

    #205166

    Purpose

    Expresses FIREmate-ECFP-Giantin in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Giantin
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL-rpb1-E1103G

    Plasmid

    #10989

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-E1103G (RPO21 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 3308 A to G in the RPB1 ORF resulting in…

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pYPQAT10

    Plasmid

    #223382

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-anti-mCherry(LaM6) PAGER(Gs)

    Plasmid

    #230011

    Purpose

    Expresses anti-mCherry PAGER(Gs) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1Dxs
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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