...Code Expansion Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible...regulate the activity of a protein so that it is responsive to specific stimuli, such as light. On a broader... the genetic code, there are several things to consider. If you are working off of a previously established...phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein...

...cell-to-cell communications and environmental sensing. Sensing and Signaling Plasmids Search the table by...Biology plasmids for Sensing and Signaling. Plasmid... Plasmid Collections Synthetic Biology Sensing and Signaling Synthetic...Synthetic Biology: Sensing and Signaling SynBio Resources SynBio Home Browse All SynBio Assembly Standards...Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection...

...Kebabian, John W., and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission...Gary L. Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain...

...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to expand...maximizing label density. In some cases, nanobodies have been found to help improve label density (Mikhaylova... Bournonville L, et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria...Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously... ExM images in a new publication.         Why Expansion Microscopy?  Traditional light microscopes are...samples can be expanded to ~4-10x linearly! This expansion physically separates molecules while maintaining...

...serve Addgene and ourselves. We are also able to consider different technical approaches that may come up...

...was aware that such roles existed, but did not consider them for myself. I started picturing myself in...technical support specialist for 4 years. Read on for insights from my time in technical support including tips...takes some time (several years) to build up comprehensive lab experience. With this valuable training ...any incoming requests or problems - there is considerable diversity in the types of questions customers...individual stories and get (sometimes refreshing) insights into how other scientists think and work. Most...drive to help others. These roles offer an easy transition from an academic lab to industry without being...

...investigator who is using an in vivo animal model should consider the possible elicited immune response from viral...

...s post, we’ll discuss some of the important considerations you should think about when developing optogenetics...place aversion also models reinforcement but is considerably simpler than ICSS. The test chamber is divided... of the type of behavior, certain technical considerations still apply. Testing stimulation First, In ...cue. The hippocampus has long been known to be responsible for mediating this behavioral effect but the ...mouse; however, all the steps and technical considerations are directly applicable to the rat [6]. Technical...PMC3150193. 8.Han, Xue, et al. "A high-light sensitivity optical neural silencer: development and application...

...Plasmid Sharing Or read more about what happens Inside Addgene   ...

...development of transient transformation systems for other species as well. Things to consider in developing...generating a transgenic plant can take even longer, a transient plant leaf transformation process could save the...transgenic plant takes even longer, is more labor intensive, inefficient, and often not reproducible. Prior...to creating transgenic cultures or plants, our transient expression method enables us to quickly screen...biosynthetic pathway. Why plant scientists do transient leaf transformations The development of transgenic...common problem in non-model organisms. Therefore, transient methods are usually the first choice for investigating...instead of months to years. In the plant sciences “transient transformation” usual means that neither a stable...

...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...meaning that the final protocol may involve a sensitive balance of trade-offs. This balance can be extremely...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

...Understanding the transition While a PhD equips you with exceptional technical skills, transitioning into management...   Strategies for a successful transition To successfully transition into a managerial role, it's essential...mentors who have made similar transitions can provide you with insights, lessons from their experiences... most pivotal decisions many PhDs face when transitioning from academia to industry is whether to stay...preparation and a willingness to embrace new responsibilities. After my second postdoc, I realized that...career trajectories within the industry, but the transition wasn’t easy - I had to reinvent myself and redefine...d like to share below. Common pitfalls in the transition (what I wish I knew before experimenting on myself...

...should be an important consideration during any experiment. Sensitivity Cas9 sensitivity is often closely related... important factors to consider: site accessibility, specificity, and sensitivity. Site accessibility Site...Another important consideration when choosing a Cas9 is balancing specificity and sensitivity. Wild-type Cas9..., accessibility, specificity, and sensitivity are key considerations in choosing a Cas protein for your...appropriate for your specific research question. Considerations when choosing your Cas nuclease When choosing... related to specificity. Sensitivity is a measure of the on-target activity of the Cas9 nuclease. This... measure of off-target activity (left) while sensitivity is the measure of on-target activity (right)....

...technique! What is a photosensitizer? First, for a few definitions. A photosensitizer is a dye or protein...system? Turns out that with light-inducible photosensitizers and a quick zap of the proper color light,...light, you can do just that.  Photosensitizing dyes and proteins have been around for awhile (check out this...Bruchez and Tsang labs recently developed a photosensitizer composed of the protein complex complex and... oxygen species (ROS). The ROS produced by photosensitizers inactivate target proteins or cells while ...having little effect on neighboring cells. The photosensitizer used in this work is composed of two parts:...fluorogen (the TAP - targeted and activated photosensitizer - MG-2I in this case). The fluorogen is not...

... as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing...tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain is a tricky organ...mm3). Current channelrhodopsins also need high-intensity light for activation which requires implantation...design new channelrhodopsins that are more light sensitive and have enhanced conductance compared to current...channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This model was then used... measured photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s... produced stronger photocurrents at low light intensity than existing channelrhodopsins CoChR and/or ChR2...

...to individual researchers. Yet accurate and comprehensible organization of data is essential for efficient...store raw data in formats that require specific (expensive) software that evolves over time such that prior... the journals themselves should be primarily responsible for storage.  If datasets are too large to transfer...projection of it, could allow idea generation and extension, based on existing, published data. The importance...However, expectations must also be reasonable, sensitive to the data type and made as facile for both the...

...and quantify based on band intensities of your DNA relative to the intensity in the ladder. It’s best to...things to consider: cost, time, equipment, and expected DNA concentration. It’s important to consider the advantages...any special reagents. However, there’s limited sensitivity at low concentrations of DNA and it can’t distinguish...measure all nucleic acids). These methods are more sensitive than UV absorbance, especially when you expect... to compare the band intensity of the fragment in the ladder that is closest in size to your piece of ...nm. This method is time consuming and has low sensitivity so it’s not used much. However, the measurements...

...and a Spanish minor, it’s time for me to start considering my future path. Talking to Addgenies has helped...biggest takeaways from my co-op. It has been so insightful to learn about different career paths, successes...

...researchers, we wondered how we could make the insights gained during this time more visible and thereby... ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video to learn ... ground again and again as they don’t save the insights they glean from a reading session. To remember...notes on a “Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save...papers to find the right experiment! The ReFigure extension also tells readers if a paper they’re reading ...ReFigure making it easy for others to discover your insights on a topic. Beyond collating other’s data, you...instantly and are editable by the author at any time. Insights contained in your ReFigure could be beneficial...