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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14 results for: NSI

Showing: 1 - 14 of 14 results

  1. Antibody Guide

    Type
    Guide
    ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions such as anaphylactic...requirements, monoclonal antibodies are typically more expensive than polyclonal antibodies. Environmental factors... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments... protein. Fluorophores Fluorophores are light-sensitive molecules which emit a specific wavelength of ...

  2. Plan Your Experiment

    Type
    Guide
    ...Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt...essentially function as a “knockout,” but be sure to consider potential consequences of having a non-functional...be no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with different PAM sequences...completely avoid them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity...to off-target activity, it is also important to consider factors that maximize cleavage of the desired ...with BioRender.com. When choosing your system, consider which Cas enzyme you are using (as species and...for your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...

  3. Optogenetics Guide

    Type
    Guide
    ... the following enhancements: Increased light sensitivity Example: ArchT Increased photocurrent amplitude... Volvox carteri 570 Chronos High-speed, light-sensitive channel from Stigeoclonium helveticum 500 Chrimson...from Halorubrum strain TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition... delivery system. Here are some key factors to consider: Optogenetic excitation or optogenetic inhibition...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...Rhodopseudomonas palustris bacteria. Key feature is the sensitivity to 740- to 780-nm near-infrared light. 740–780...488 LOV domains LOV-based Light-oxygen-voltage-sensing (LOV) domains are protein sensors used by many ...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...the viral genetic material inside. The AAV genome is ~4.7 kb long and consists of two open reading frames...ITRs is limited by the physical space available inside of the small AAV capsid and directly correlates...Genome Integration Recombinant AAVs are generally considered to not integrate into the host genome. When wild-type...during production, as it carries the Cap region responsible for the production of capsid proteins. AAV2 is...it was the first to be well-characterized and extensively studied, and has a broad tissue tropism. Table...information. Self-complementary AAV (scAAV) One downside of the use of AAVs as viral vectors is their single-stranded...common points of entry. Biosafety should always be considered with respect to the precise nature of experiments...

  5. Chemogenetics Guide

    Type
    Guide
    ...molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands and are engineered to respond... development of mutated receptors that while unresponsive to endogenous ligands, could be activated by... coupled receptors, but unlike RASSLs, show insensitivity to endogenous ligands, have low constitutive...proteins of a light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate...luciferase-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs with increased...chemogenetics experiment, here are some things to consider: Chemogenetic activation or inhibition. Do you... a study in rats using an hM4Di receptor might consider an alternative DREADD ligand such as DCZ versus...

  6. CRISPR Guide

    Type
    Guide
    ...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...There are two main types of base edits: Base transitions - edit to same classification of nucleotide base...pyrimidine-to-pyrimidine), which catalyze base transitions. Cytosine base editors are created by fusing ...integrated along with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases... target gene function. It has also been used extensively to screen for novel genes that regulate known...simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single transcriptional...silencing across much of the human genome following transient expression of the dCas9 fusion, with the epigenetic...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...plasmids (mostly third-generation) can be used in transient transfections, but expression will be greatly ...plasmids cannot be used in direct transfections for transient expression of a transgene. For more detailed protocols...stable expression and wide tropism. Addgene has an extensive collection of lentiviral CRISPR libraries, including...initial infection. Third-generation systems are considered safer than second-generation systems because ...transfer plasmid is an oncogene. Additional consideration for oncogenes should be made on a case-by-case...common points of entry. Biosafety should always be considered with respect to the precise nature of experiments...9765382 Durand, S., & Cimarelli, A. (2011). The inside out of lentiviral vectors. Viruses , 3 (2), 132...

  8. Adenovirus Guide

    Type
    Guide
    ...a protein capsid and the viral genetic material inside. Adenoviral genomes range between 26–45 kb in length...ITRs is limited by the physical space available inside of the adenoviral capsid and directly correlates...used generation for research purposes. The system consists of two plasmids that eventually recombine to form...expression delivered through rAdV vectors is normally transient and short-lived. Second- and third-generation ...common points of entry. Biosafety should always be considered with respect to the precise nature of experiments...the Adenoviridae family. Episome Genetic element inside the cell that remains as a part of the eukaryotic...

  9. Promoters

    Type
    Guide
    ...equivalent called the Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter...position, simply titled the -35 element, typically consists of the sequence TTGACA and this element controls...have also been discovered in eukaryotes. Operons consist of a promoter, which is recognized by the RNA polymerase...transcribes messenger RNA (mRNA) which is the RNA responsible for providing a stable template for the translation... RNAs (tRNA), the adaptor molecules that are responsible for bringing amino acids to the ribosome when...

  10. Modular Cloning Guide

    Type
    Guide
    ...gene-editing tools, and more. The MoClo system consists of three sets of cloning vectors (Level 0, 1, ... for a variety of applications. EcoFlex MoClo Extension, Volume I Bacterial Expression Mikhail Shapiro...and expression tuning in E. coli . CIDAR MoClo Extension, Volume I Bacterial Expression Richard Murray ...used with plasmids from the MoClo-YTK . EMMA: Extensible Mammalian Modular Assembly Toolkit Mammalian ... to generate mammalian expression vectors for transient or stable transfections. PUF Assembly Kit Mammalian...

  11. Molecular Biology Reference

    Type
    Guide
    ...additional strain-related information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen...sequencing chromatogram which provides the color and intensity of each fluorescent signal. Sanger can sequence...and C always pairs with G. RNA on the other hand consists of Adenine, Cytosine, Guanine and Uracil (U). ...

  12. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...transfer plasmid is an oncogene. Additional consideration for oncogenes should be made on a case-by-case...common points of entry. Biosafety should always be considered with respect to the precise nature of experiments...

  14. Cloning

    Type
    Guide
    ...vectors and recombination enzymes can be quite expensive. In addition it is quite difficult to switch to...
Showing: 1 - 14 of 14 results