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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 92 results for: NSI

Showing: 1 - 20 of 92 results

  1. Genetic Code Expansion

    Type
    Collection
    ...Code Expansion Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible...regulate the activity of a protein so that it is responsive to specific stimuli, such as light. On a broader... the genetic code, there are several things to consider. If you are working off of a previously established...phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein...

  2. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...cell-to-cell communications and environmental sensing. Sensing and Signaling Plasmids Search the table by...Biology plasmids for Sensing and Signaling. Plasmid... Plasmid Collections Synthetic Biology Sensing and Signaling Synthetic...Synthetic Biology: Sensing and Signaling SynBio Resources SynBio Home Browse All SynBio Assembly Standards...Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring mechanical tension across vinculin...7303):263-6. Martin Schwartz Tension Talin tension sensors Talin tension sensor reveals novel features...F40-based tension sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension... tension sensors Tunable molecular tension sensors reveal extension-based control of vinculin loading.... cAMPFIRE cAMP biosensors with improved sensitivity Sensitive genetically encoded sensors for population...highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like...transmission and mechanosensitivity. J Cell Biol. 2016 May 9;213(3):371-83. Martin Schwartz Tension HP35-, FL...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Amber...linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus VCA Heterotrimeric...Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct...construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct consisting... linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean-5aa linker-Venus... linker-Amber VCAA Heterotetrameric construct consisting of Venus-5aa linker-Cerulean-5aa linker-Amber... linker-Amber VCVV Heterotetrameric construct consisting of Venus-5aa linker-Cerulean-5aa linker-Venus...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...type of transduction (transient or stable) may depend on the desired sensitivity, level of induction, ...Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids Inducible and repressible promoters...has been modified to provide two classes of tet-responsive systems: Tet-Off and Tet-On. Read on to learn...components of these systems for better efficiency, sensitivity, and control of expression. These include: Tet-On...rtTA-Advanced, also known as rtTA2 S -M2) with greater sensitivity to doxycycline and three copies of a minimal ...variants with lower background activity and greater sensitivity to doxycycline (Zhou et al., 2006). Tet-On 3G...interactions with endogenous factors. Experimental Considerations Expression System If your gene of interest ...

  6. Antibody Guide

    Type
    Collection
    ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions such as anaphylactic...requirements, monoclonal antibodies are typically more expensive than polyclonal antibodies. Environmental factors... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments... protein. Fluorophores Fluorophores are light-sensitive molecules which emit a specific wavelength of ...

  7. Plan Your Experiment

    Type
    Collection
    ...Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt...essentially function as a “knockout,” but be sure to consider potential consequences of having a non-functional...be no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with different PAM sequences...completely avoid them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity...to off-target activity, it is also important to consider factors that maximize cleavage of the desired ...with BioRender.com. When choosing your system, consider which Cas enzyme you are using (as species and...for your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ... proteins consists of Type I, Type II, and Type III interferons. They trigger the defensive responses ..., WBCQ1 DEFA1 defensin, alpha 1 DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS DEFA3 defensin, alpha 3, neutrophil-specific..., HP-3 DEFA5 defensin, alpha 5, Paneth cell-specific DEF5, HD-5, MGC129728 DEFB1 defensin, beta 1 BD1,...MGC51822 DEFB103A defensin, beta 103A DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3 DEFB104A defensin, beta 104A ...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...erythroleukemia (MEL) cells, a suspension cell line. The culture medium in all steps consists of DMEM supplemented...eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool that...off-target effects). Ensure that the guide sequences consist of a 20-mer (“protospacer sequence”) upstream of..., resolve agarose gel to determine if size is consistent with the presence or absence of deletion. For...L-glutamine, which is used for MEL cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully...strategies for each cell type. While MEL cells are suspension cells, instructions for adherent cells have ...validation (see step 6 ). NOTE: This protocol is for suspension cells. Adherent cells can either grow as individual...

  10. COVID-19 Resources

    Type
    Collection
    ...receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another key step is the priming of ...to identify antibody escape mutants and for comprehensive mapping. Some of the following plasmids are ...the links and references below: ACE2 - (Human Angiotensin Converting Enzyme 2) is the host cell receptor...SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably detect nucleic acids at...and optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2...available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for... Library of Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor...

  11. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...MCP tagged plasmids for visualization of proteins inside cells....ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated ...visit the New England Biolabs website. For a comprehensive comparison to GFP, please refer to NEB's comparison...Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control...Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control...pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector...Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...plasmid backbone to use, you have many elements to consider. Here is a guide to a selection of Addgene's empty...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian...gene with N-terminal GST tag and TEV cleavage Transient expression - Gradia Lab plasmids for bacterial...EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging...other common uses. Viral Vector Delivery Although transient expression is sufficient for some experiments,...integrate into the host genome Addgene has an extensive collection of plasmids for packaging and expression...Lentiviral backbones made by Addgene Addgene has an extensive collection of plasmids for packaging and expression...

  13. Bacterial Expression Systems

    Type
    Collection
    ...Genome Engineering You may also like… Genetic Code Expansion Collection Microbiology Plasmids Collection Synthetic... Consortium (SGC) collection page contains an extensive selection of plasmids and tags for protein purification...Visit our Fluorescent Protein Guide to find an extensive collection of FPs, including photoactivatable,...for non-toxic protein labeling and visualization inside living or fixed bacterial and mammalian cells. ...Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia coli Cynthia Collins 84689...Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing molecule) Escherichia coli Cynthia Collins 112197... (Tet) Inducible Expression Collection for an extensive selection of plasmids using tetracycline-regulated...

  14. AAV Packaged on Request

    Type
    Collection
    ...Viral vector production Density centrifugation purification Addgene’s comprehensive quality control process... amplify DNA, produce the prep, and perform comprehensive quality control processes to make sure it is...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists... that complement published data, capturing the insights that often go unshared. Help Center Check out ...

  15. CRISPR Guide

    Type
    Collection
    ...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...There are two main types of base edits: Base transitions - edit to same classification of nucleotide base...pyrimidine-to-pyrimidine), which catalyze base transitions. Cytosine base editors are created by fusing ...integrated along with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases... target gene function. It has also been used extensively to screen for novel genes that regulate known...simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single transcriptional...silencing across much of the human genome following transient expression of the dCas9 fusion, with the epigenetic...

  16. Viral Vector Guides and Plasmids

    Type
    Collection
    ...capacity, high transduction efficiency, and high but transient transgene expression.They can induce a strong ...RNA < 8 kb 80–130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb ...Dividing/Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing...divided into multi-plasmid systems. These systems consist of a plasmid containing the genetic information...

  17. Promega Plasmid Collection

    Type
    Collection
    ...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...collection contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors... measure subtle expression changes with high sensitivity. These assays are ideal for real-time monitoring...bright and stable luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc...

  18. Luciferase Plasmid Collection

    Type
    Collection
    .... pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin), a pH-stable... Plasmid Collection Luciferase is the enzyme responsible for bioluminescence found in a variety of organisms... the emission of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching...

  19. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Plasmids MORF Pooled Library The MORF library is a comprehensive human transcription factor collection that contains...Pooled Library The MORF pooled library is a comprehensive collection of human transcription factor open...

  20. Synthetic Biology - Overview

    Type
    Collection
    ...as logic gates and higher level gene networks. Sensing and Signaling Browse plasmids related to intercellular...intercellular signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial...Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB...
Showing: 1 - 20 of 92 results