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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Publication

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Publication

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-G2304D

Plasmid

#182845

Purpose

mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

Depositor

Peter Harte

Publication

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Tags

GST

Expression

Bacterial

Mutation

mutation G2304D (GGC/GAC)

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmPop2a-dsRNAres-V5His6_K

Plasmid

#146717

Purpose

Insect Expression of DmPop2a-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmPop2a-dsRNAres (Pop2 Fly)

Expression

Insect

Mutation

ORF extracted from CG5684-Pa; NM_140281.2, with o…

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D

Plasmid

#146121

Purpose

Insect Expression of DmEDC3_1-101

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-101 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D

Plasmid

#146122

Purpose

Insect Expression of DmEDC3_1-338

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-338 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPumilio_B

Plasmid

#145945

Purpose

Insect Expression of DmPumilio

Depositor

Elisa Izaurralde

Insert

DmPumilio (pum Fly)

Expression

Insect

Mutation

three silent and one non silent mutation and a de…

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub+DsxF].1046A

Plasmid

#112694

Purpose

express two gRNA targeting bTub & DsxF under dU6-3 promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG-STII PLU-HIS

Plasmid

#112993

Purpose

Baculovirus expression of Drosophila png-Flag and plu-His

Depositor

Terry Orr-Weaver

Inserts

png (png Fly)
plu (plu Fly)

Tags

Flag and His

Expression

Insect

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F04

Plasmid

#71030

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sin3A (Sin3A Fly)

Use

Gateway Cloning

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00050

Plasmid

#34212

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11502 (svp Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00060

Plasmid

#34221

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9415 (Xbp1 Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Khc73(1-387)-GFP

Plasmid

#31747

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-387

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
Khc73(1-432)-GFP

Plasmid

#31748

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-432

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
CJ47 (U1 snRNA (U1;21d) 3G mutant)

Plasmid

#17776

Depositor

David Bartel

Publication

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

U1 snRNA (U1;21d) (snRNA:U1:21D Fly)

Mutation

3G mutant

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pNos-myrGFP

Plasmid

#253595

Purpose

Express myrGFP under Drosophila nanos promoter

Depositor

Mayu Inaba-Oguro

Publication

Bener et al Commun Biol. 2026 Mar 9;9(1):556. doi: 1

Insert

myrGFP

Tags

myristoylation signal

Expression

Insect

Promoter

Nanos promoter

Available since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT1AR

Plasmid

#221820

Purpose

Plasmid to express gRNA (gaccagtccactaccgcagc) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Publication

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1AR gRNA (5-HT1A Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-SoxN-A22-EGFP

Plasmid

#181731

Purpose

Galactose inducible expression of SoxN-A22-EGFP

Depositor

Alejandro Chavez

Insert

SoxN-A22-EGFP (SoxN Fly)

Tags

3xFLAG-EGFP

Expression

Yeast

Mutation

extended repeat of 22 Ala residues replacing nati…

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pKC52

Plasmid

#226780

Purpose

Expresses Drosophila UNC-104(R348G) in insect cells

Depositor

Kyoko Chiba

Publication

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R348G) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 348 to Glycine

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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