We narrowed to 15,088 results for: pET

1. pET-Galectin-8 linker deletion

   Plasmid

   #68806

   Purpose

   Express Galectin-8 linker deletion protein in Bacterial cells

   Depositor

   Hyun Kyu Song

   Article

   Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

   Insert

   Galectin-8 (LGALS8 Human)
   

   Tags

   N-terminal 6-histidine-tag

   Expression

   Bacterial

   Mutation

   No mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28-MRPP3-myc-His

   Plasmid

   #67864

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   MRPP3 (KIAA0391 Human)
   

   Tags

   His and myc

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b/EYFP-GgVcl 1-851

   Plasmid

   #46264

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

   Insert

   Vcl 1-851 (VCL Chicken)
   

   Tags

   EYFP and His

   Expression

   Bacterial

   Mutation

   aa 1-851 (aka Vh) head domain

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-DEUT RUVBL1 Delete

   Plasmid

   #159144

   Purpose

   Expression and protien purification of mutated RUVBL1

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Expression

   Bacterial

   Mutation

   amino acid 1-463, Glu134-Glu237 mutated to GPPG

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a(+)-MVK mazei

   Plasmid

   #108947

   Purpose

   heterologous expression of MVK of M. mazei in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Mevalonate kinase (MVK)
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-22b(+)-RNase HIII

   Plasmid

   #12683

   Depositor

   SeWon Suh

   Article

   Kwak et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   RNase HIII
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-His6-Ubc9-C138A

   Plasmid

   #133911

   Purpose

   Bacterial expression of His6-Ubc9-C138A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 138 to Alanine

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-DEST42-SARA-V5-His6

   Plasmid

   #67431

   Purpose

   Expression of hSara with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Tags

   His and V5

   Expression

   Bacterial

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid

    #84634

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsub(TA)DD-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-Fab1 (Francisella tularensis)

    Plasmid

    #61690

    Purpose

    Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

    Depositor

    Michael Johnson

    Article

    Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

    Insert

    enoyl-[acyl-carrier-protein] reductase (NADH)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET-PLS-KP6-A66S_rpoA-U

    Plasmid

    #190969

    Purpose

    Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-PLS-KP6-E67A_rpoA-U

    Plasmid

    #190973

    Purpose

    Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-pro-siRNA-V2-LMNA

    Plasmid

    #111088

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    Lamin A/C (LMNA Human)
    

    Expression

    Bacterial

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-TAF4(827-909)

    Plasmid

    #105620

    Purpose

    bacterially express TAF4 HFD

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF4-HFD(827-909) (Taf4 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

    Plasmid

    #184406

    Purpose

    yeast surface display of the SARS-CoV-2 Beta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    K417N+E484K+N501Y

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid

    #184408

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pet22b-mCherry-TEAD4-IDR

    Plasmid

    #166442

    Purpose

    Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, TEAD4-IDR (TEAD4 Human)
    

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b/GgVcl V1-851 (aka Vh)

    Plasmid

    #46172

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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