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HCT55_rAAV-eHGT453m_eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA

Plasmid

#223945

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hPGK-DEST-IRES-EGFP

Plasmid

#253774

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-TRE-DEST-UbC-tTSrtTA-IRES-Hygro

Plasmid

#253779

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-EV-IRES-Neo

Plasmid

#253787

Purpose

pLV lentiviral expression vector encoding empty vector linked to neomycin selection by an IRES under a human EF1alpha promoter.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UbC-DEST-IRES-Puro

Plasmid

#253777

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TRE-DEST-UbC-tTSrtTA-IRES-Neo

Plasmid

#253780

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UbC-DEST-IRES-Neo

Plasmid

#253776

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-UbC-DEST-IRES-EGFP

Plasmid

#253778

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TRE-DEST-UbC-tTSrtTA-IRES-EGFP

Plasmid

#253782

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1alpha-DEST-IRES-Hygro

Plasmid

#253767

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1alpha-DEST-IRES-Neo

Plasmid

#253768

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TRE-DEST-UbC-tTSrtTA-IRES-Puro

Plasmid

#253781

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a Tet-On inducible promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pROM-POU3F2p-mCherry-Neo

Plasmid

#153321

Purpose

Expresses mCherry from the POU3F2 promoter

Depositor

Robert Judson-Torres

Article

Zhang et al Cell Rep. 2025 Dec 23;44(12):116675. doi

Insert

POU3F2 Promoter (POU3F2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

N/A

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-MerCreMer-hygro

Plasmid

#188982

Purpose

Retroviral expression of tamoxifen-inducible Cre recombinase

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

Tamoxifen-inducible Cre recombinase

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-sgTrp53-sgAtrx-EFS-Cre

Plasmid

#189977

Purpose

Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

sgTrp53, sgAtrx, Cre recombinase

Use

AAV, Cre/Lox, and Mouse Targeting

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HCT159_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

Plasmid

#233558

Purpose

Direct-expressing iCre297T AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

iCre297T

Use

AAV

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HCT160_pAAV-3X-eHGT_1139m-minBGprom-iCre297T-WPRE3-bGHpA

Plasmid

#233559

Purpose

Direct-expressing iCre297T AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

iCre297T

Use

AAV

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HCT46_rAAV-eHGT_1188m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#223943

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HCT49_rAAV-3Xcore-eHGT_1139m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#223944

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1alpha-FLAG-HA-CAD_S1900A-HA-IRES-Neo

Plasmid

#253789

Purpose

pLV lentiviral expression vector encoding FLAG- and HA-tagged human CAD bearing S1900A mutation linked to neomycin selection by an IRES under a human EF1alpha promoter.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Insert

CAD (CAD Human)

Use

Lentiviral

Tags

FLAG-HA and HA

Expression

Mammalian

Mutation

S1900A

Promoter

EF1alpha

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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