We narrowed to 4,060 results for: gast

1. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

   Plasmid

   #201683

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111545

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChrimson_tdtomato_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. human gamma-catenin pcDNA3

   Plasmid

   #16827

   Depositor

   Eric Fearon

   Article

   Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

   Insert

   gamma-catenin (JUP Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. BSD-P2A-TagRFP-hSmad1-donor

   Plasmid

   #203662

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

   Insert

   smad1 (SMAD1 Human)
   

   Tags

   RFP

   Expression

   Mammalian

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHD-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199204

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3xFLAG-CMV14/p14ARF

   Plasmid

   #78760

   Purpose

   To overexpress p14ARF in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

   Insert

   ARF (CDKN2A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

7. 20XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111546

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABR IRFP-Bcd-LEXY

   Plasmid

   #182595

   Purpose

   Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Tags

   IRFP and LEXY

   Expression

   Insect

   Promoter

   maternal tubulin

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BII-ChBtW-IGF2

    Plasmid

    #133374

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IGF2 (IGF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His Puc (aa 1-393)

    Plasmid

    #52295

    Purpose

    Bacterial expression of His tagged Drosophila Puc fragment

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    Puc (puc Fly)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

12. p55-H2B-Dendra2

    Plasmid

    #80609

    Purpose

    Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    H2B-Dendra2 (H2BC21 Synthetic, Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-ChiNW-IGF2

    Plasmid

    #133383

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IGF2 (IGF2 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 10XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111547

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Tags

    3x HA, V5

    Expression

    Insect

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS5-3HA/p14ARF

    Plasmid

    #78764

    Purpose

    To overexpress p14ARF in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    p14ARF (CDKN2A Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only