We narrowed to 26,176 results for: vit;

1. pcDNA3.1-PDGFRB-V5/HIS

   Plasmid

   #201988

   Purpose

   expression of human PDGFRB receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRB receptor tyrosine kinase (PDGFRB Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKrox24(2xD-E_inD)Luc

   Plasmid

   #200109

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element in front of EGR1 promoter…

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-pmExRai-AKAR2

   Plasmid

   #161755

   Purpose

   Plasma membrane-targeted ExRai-AKAR2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   Lyn-ExRai-AKAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV EF1a DIO iChloC 2A dsRed

   Plasmid

   #70762

   Purpose

   An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E83Q,E90R,E101S,D156N,T159C

   Promoter

   EF1a

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mitoExRai-AKAR2

   Plasmid

   #161757

   Purpose

   ExRai-AKAR2 targeted to the outer mitochondrial membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   DAKAP-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKrox24(MapErk)Luc

   Plasmid

   #200112

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence in minimal p…

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-erExRai-AKAR2

   Plasmid

   #161756

   Purpose

   ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   CYP450-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKrox24(MapErk)dTomato

   Plasmid

   #200113

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence, no other pr…

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TK-GFP-Nluc-P2A-neo-TK

   Plasmid

   #134896

   Purpose

   Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Insert

   TK-GFP-Nluc-P2A-Neo-TK
   

   Use

   Cryptosporidium expression

   Tags

   Expression

   Mutation

   Promoter

   Cryptosporidium actin and enolase

   Available Since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKrox24(2xD-E)dTomato

    Plasmid

    #200110

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. delCMV-mCherry-3x-p67phox-GBD

    Plasmid

    #214141

    Purpose

    Rac activity sensor

    Depositor

    Leif Dehmelt

    Article

    Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

    Insert

    3xp67phox-GBD (NCF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only the GTPase binding domain (GBD) is included

    Promoter

    delCMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-ExRai-AKAR2(T/A)

    Plasmid

    #161754

    Purpose

    Negative-control mutant for ExRai-AKAR2 biosensor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2(T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKrox24(MapErk)DsRed

    Plasmid

    #200114

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-RET-V5/HIS

    Plasmid

    #205606

    Purpose

    Expression of human RET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RET receptor tyrosine kinase (RET Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DDX41-GFP

    Plasmid

    #175494

    Purpose

    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.

    Depositor

    Evan Braunstein

    Article

    Li et al Haematologica. 2016 Jun;101(6):e228-31.

    Insert

    DDX41 (DDX41 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.3 HA-hTSPAN12

    Plasmid

    #115784

    Purpose

    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    TSPAN12 (TSPAN12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    multiple silent mutations introduce restriction s…

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/V5-His-mPer1

    Plasmid

    #219421

    Purpose

    Expresses 3873 bp of mPer1 coding sequence in E. coli.

    Depositor

    Aziz Sancar

    Article

    Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mPer1 (Per1 Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Syn-ATP

    Plasmid

    #51819

    Purpose

    Optical reporter of presynaptic ATP

    Depositor

    Timothy Ryan

    Article

    Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

    Insert

    Chimeric Synaptophysin-mCherry-Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Within WT luciferase gene, changed Threonine 214 …

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZW12 (CK2beta)

    Plasmid

    #27088

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZW16 (CK2alpha')

    Plasmid

    #27087

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' (CSNK2A2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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