We narrowed to 10,374 results for: plasmids 101

1. pGL3-noSV40-humanEC1.25_S3-4-5-6

   Plasmid

   #173956

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

   Plasmid

   #212504

   Purpose

   Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

   Plasmid

   #207493

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-851_HA-GD2-28z_HA-TFAP4

   Plasmid

   #207501

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK-HA-PGRN

   Plasmid

   #234872

   Purpose

   Lentiviral plasmid expressing HA-tagged human progranulin.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   Progranulin (GRN Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-U6-DR30(SapI)-EFS-CasRx-pA

   Plasmid

   #192488

   Purpose

   To express CasRX and a gRNA

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   EFS

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EFS-DjCas13d-HA-mCherry-pA

   Plasmid

   #233030

   Purpose

   To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   DjCas13d-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-GFP-pA

   Plasmid

   #192500

   Purpose

   To express DjCas13d compatible gRNA and GFP

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   DjCas13d
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   CMV

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV U6-DR30(sapI)-0.5Syn-CasRX-mCherry-pA

   Plasmid

   #192489

   Purpose

   To express CasRX and a gRNA

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   0.5Syn

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV U6-DR30(sapI)-0.4CaMKIIa-CasRX-mCherry-pA

    Plasmid

    #192490

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    0.4CaMKIIa

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-U6-DR30(SapI)-0.5Syn-CasRx-pA

    Plasmid

    #192492

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    0.5Syn

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-U6-DR30(SapI)-CMV-intron-GFP-pA

    Plasmid

    #192493

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    CMV

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EFS-hfCas13d-T2A-mCherry-pA

    Plasmid

    #192497

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGgaatacattaccaacGTGGTGtacGTG

    Promoter

    EFS

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-U6-DR30(SapI)-CMV-intron-CasRx-pA

    Plasmid

    #192485

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    No

    Promoter

    CMV

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EFS-hfCas13d-HA-mCherry-pA

    Plasmid

    #233029

    Purpose

    To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    hfCas13d-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EFS-CasRx-HA-mCherry-pA

    Plasmid

    #233028

    Purpose

    To Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

    Plasmid

    #233031

    Purpose

    To express a DjCas13d-compatible gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    DjCas13d-compatible gRNA expression cassette
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EFS-CasRx-P2A-mCherry-pA

    Plasmid

    #233025

    Purpose

    To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx/mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-intron-hfCas13d-pA

    Plasmid

    #195864

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CMV-intron-DIO-hfCas13d-pA

    Plasmid

    #195866

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge