We narrowed to 4,060 results for: gast

1. Dox-Akt

   Plasmid

   #207328

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   rac protein kinase-alpha (AKT1 Human)
   

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 129 - 480

   Promoter

   Tet Response Element

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. KHBD00476

   Plasmid

   #39585

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5838 (Dref Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

   Plasmid

   #201686

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. UAS-dsarm(E)

   Plasmid

   #187870

   Purpose

   Gal4/UAS expression of dSarm(isoE)

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   dSarm isoform E (Sarm Fly)
   

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hy_pMT Laccase2 Exons 1-3

   Plasmid

   #91799

   Purpose

   Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

   Insert

   Laccase2 (stw Fly)
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

   Plasmid

   #79259

   Purpose

   Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   DmeIF4G (eIF4G1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   V5-His6 and lambdaN-HA

   Promoter

   Ac5 promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTYF-PRSx8-AstR-p2A-GFP

   Plasmid

   #159632

   Purpose

   Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

   Depositor

   Andrew Allen

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   AstR (AstA-R1 Fly)
   

   Use

   Lentiviral

   Tags

   GFP and P2A

   Expression

   Mammalian

   Promoter

   PRSx8

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-GFP-dSUMO

   Plasmid

   #85696

   Purpose

   expression of GFP-SUMO1 in Drosophila cells

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   SUMO (Sumo Fly)
   

   Tags

   GFP

   Expression

   Insect

   Promoter

   Ac5

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

9. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-Brn2-DBD-EnR

    Plasmid

    #19714

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Fly engrailed 36240 (Pou3f2, en Fly, Mouse)
    

    Expression

    Mammalian

    Mutation

    mouse Brn2 DNA binding domain-Drosophila engraile…

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

11. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid

    #196063

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    

    Expression

    Worm

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1 IntS6 AA 1035-1284

    Plasmid

    #196904

    Purpose

    Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 6 (IntS6 Fly)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. KHBD00044

    Plasmid

    #34206

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17117 (hth Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pRSFDuet-1 IntS11 AA 300-597

    Plasmid

    #199329

    Purpose

    Expresses His-tagged Drosophila IntS11 amino acids 300-597

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 11 (IntS11 Fly)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 300-597 only

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LN-entry+NLS

    Plasmid

    #128011

    Purpose

    Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    LN-3xFlag-SV40NLS
    

    Use

    Lamdan entry vector with nls to generate tetherin…

    Tags

    LN-3xFlag-SV40NLS

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. 20XUAS IVS Jaws mCherry_tr

    Plasmid

    #111549

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS3-MT-BX/p14ARF

    Plasmid

    #78761

    Purpose

    To overexpress p14ARF in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Tags

    6xMYC

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMT-Pav-GFP

    Plasmid

    #24286

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Pavarotti (pav Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pBS-Squ:mCherry

    Plasmid

    #20163

    Depositor

    Eric Wieschaus

    Article

    Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

    Insert

    spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
    

    Expression

    Bacterial

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

20. Nup98-pDONR221

    Plasmid

    #124241

    Purpose

    Gateway entry clone of Drosophila Nup98-96 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Nup98-96 (Nup98-96 Fly)
    

    Expression

    Insect

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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