We narrowed to 159,237 results for: addgene

1. Venus-Bik-GG153AA-pEGFP-C1

   Plasmid

   #177427

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Glycines 153 and 154 mutated to alanine

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-Bik-G154STOP-pEGFP-C1

   Plasmid

   #177428

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Glycine 154 replaced with STOP codon

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. NODAL-T7TS

   Plasmid

   #115250

   Purpose

   For in vitro transcription of human NODAL open reading frame RNA

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NODALvar-T7TS

   Plasmid

   #115251

   Purpose

   For in vitro transcription of a human NODAL splice variant open reading frame RNA

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL splice variant (NODAL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. H2B-MiniE-mEm

   Plasmid

   #181991

   Purpose

   MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   H2B-MiniE-mEm
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6DUB (LOC149281)

   Plasmid

   #119967

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   LOC149281 (METTL11B Human)
   

   Use

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 58-278

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. FOXN1 (FOXN1A-c017)

   Plasmid

   #110249

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   FOXN1 (FOXN1 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5T1G (CBX1)

   Plasmid

   #101884

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX1 (CBX1 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. 2QO2 (EPHA3)

   Plasmid

   #101271

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA3 (EPHA3 Human)
   

   Use

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDHJS4 AN-

    Plasmid

    #78247

    Purpose

    One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

    Inserts

    attB
    attP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only