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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor-R rich

Plasmid

#225478

Purpose

Express mEGFP-GAPDH-Tudor-R fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL S481D

Plasmid

#195224

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

S481D

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-T17N

Plasmid

#214289

Purpose

Express dominant negative Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative Cdc42 (CDC42 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-T17N

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-human p53 1-50 (HK180)

Plasmid

#206458

Purpose

p53 1-50 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

p53 1-50 (TP53 Human)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_FANCM_K819R

Plasmid

#221380

Purpose

Expresses FANCM_K819R in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Bacterial

Mutation

Changed lysine 819 to arginine

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-K22R

Plasmid

#220293

Purpose

mammalian expression of Bcl-2-K22R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

K22R

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G101V

Plasmid

#220295

Purpose

mammalian expression of Bcl-2-G101V tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

G101V

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-K17R

Plasmid

#220292

Purpose

mammalian expression of Bcl-2-K17R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

K17R

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-F104C

Plasmid

#220296

Purpose

mammalian expression of Bcl-2-F104C tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

F104C

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-T132L

Plasmid

#220303

Purpose

mammalian expression of Bcl-2-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

T132L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-A131L

Plasmid

#220302

Purpose

mammalian expression of Bcl-2-A131L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

A131L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G128L

Plasmid

#220301

Purpose

mammalian expression of Bcl-2-G128L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CG1358

Plasmid

#218541

Purpose

Drosophila CG1358 cDNA (human codon optimized)

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CG1358 (HisT Fly)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_EGFR-NTEV-tcs-GV-2xHA

Plasmid

#214610

Purpose

EGFR receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

EGFR-NTEV (EGFR Human)

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA

Plasmid

#214615

Purpose

HTR2A receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

HTR2A-NTEV (HTR2A Human)

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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