We narrowed to 7,077 results for: pcdna3.1

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pCDNA3.1-FLAG-CLIP(Q149TAG)-SNAP-StrepTag

Plasmid

#248185

Purpose

For the mammalian expression and non-cannonical labelling of FLAG-CLIP(Q149TAG)-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Publication

Smith et al Adv Sci (Weinh). 2026 Jul 21:e76672. doi

Insert

FLAG-CLIP(Q149TAG)-SNAP-StrepTag

Use

1257

Tags

FLAG, CLIP and SNAP, StrepTag

Expression

Mammalian

Mutation

Q149TAG

Promoter

CMV & T7

Available since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-P2A-eGFP

Plasmid

#239769

Purpose

Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and eGFP in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Expression

Mammalian

Mutation

K1435TAG, Y371C

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-No UTRs AAA Mutant (pcDNA3.1)

Plasmid

#238430

Purpose

Expresses GRP78 AAA mutant without the UTRs fused to FLAG tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The UTRs of the gene have been deleted. The PPP t…

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)

Plasmid

#238429

Purpose

Expresses GRP78 Full Length without UTRs fused to the FLAG Tag

Depositor

Amy Shiu Lee

Publication

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The UTRs of the gene have been deleted.

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

Plasmid

#233065

Purpose

Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Publication

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Leucine 928 to Alanine; Methionine 929 to Alanine

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[G249D]-P2A-Electra1 (pAH10)

Plasmid

#227615

Purpose

Overexpression of SLC25A46 with G249D mutation in mammalian cell lines

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

G249D

Promoter

CMV, T7

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S91, 262 ,331, 724A

Plasmid

#224738

Purpose

For expression of HA-tagged GTSE1 protein with S91, 262 ,331, 724A mutation

Depositor

Michele Pagano

Publication

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 91, 262 ,331, 724 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

Plasmid

#227617

Purpose

Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R340C

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

Plasmid

#227618

Purpose

Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

I349D

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

Plasmid

#187725

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-380

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

Plasmid

#187726

Purpose

Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-491

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

Plasmid

#215900

Purpose

Expression of TNRC6A-CIM1 domain with S1332A mutation

Depositor

Kumiko Ui-Tei

Publication

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1332A

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

Plasmid

#215901

Purpose

Expression of TNRC6A-CIM1 domain with S1346A mutation

Depositor

Kumiko Ui-Tei

Publication

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1346A

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A

Plasmid

#215903

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Publication

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A

Plasmid

#215904

Purpose

Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Publication

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1616A, S1691A

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H, P29S)

Plasmid

#224282

Purpose

Cell transfection and expression of Rac1 (G12C, K96H, P29S)

Depositor

Kevan Shokat

Publication

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C, K96H, P29S

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Publication

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Publication

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Expression

Mammalian

Mutation

Thr1273Asp substitution

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

Plasmid

#218463

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)

Depositor

Syed Saif Hasan

Publication

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )

Expression

Mammalian

Mutation

Lys1269Ala and His1271Ala substitutions

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔST-FLAG

Plasmid

#205623

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Publication

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted ST domain

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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