We narrowed to 9,913 results for: Uty

1. pCRISPR2_wt

   Plasmid

   #149386

   Purpose

   P. aeruginosa PA14 CRISPR2 locus

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. membrane-HsPLK1-dead

   Plasmid

   #185572

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   PLK1-dead (PLK1 Human)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   membrane targeting sequence from Neuromodulin (ML…

   Expression

   Mutation

   K82R kinase-dead mutant

   Promoter

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

   Plasmid

   #183387

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   redesigned Caspase-4 (AA94-377) (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   T7

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-IRES-GFP-mouse-Caspase-1-H340D

   Plasmid

   #183362

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 H340D (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   H340D

   Promoter

   MESV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

   Plasmid

   #183364

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4a (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   H342D_K345M_K379T

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-IRES-GFP-dog-Caspase-1/4b

   Plasmid

   #183365

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4b (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-IRES-GFP-dog-Caspase-1/4a

   Plasmid

   #183363

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4a (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pN1-TOMM20-Halo

   Plasmid

   #129348

   Purpose

   Halo Tag, Mitochondrial outer membrane localization

   Depositor

   Yasushi Okada

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   Halo (Promega)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1α -NWM-Node 1

   Plasmid

   #154253

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 1 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1α -NWM-Node 2

    Plasmid

    #154254

    Purpose

    expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    reconstructed Node 2 of New World monkey retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1α -NWM-Node 3

    Plasmid

    #154255

    Purpose

    expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    reconstructed Node 3 of New World monkey retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. gSCPL35-mRFP

    Plasmid

    #181960

    Purpose

    SCPL35 protein tagged with mRFP, under control of native promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    SCPL35
    

    Use

    Tags

    mRFP

    Expression

    Plant

    Mutation

    Promoter

    native SCPL35

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

    Plasmid

    #178074

    Purpose

    Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    MZT2A (MZT2A Human)
    

    Use

    Tags

    PreScission-mEGFP and ZZ-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCm N-term SMT3 NikJC199U

    Plasmid

    #174210

    Purpose

    Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCm C-term SMT3 NikJC199U

    Plasmid

    #174362

    Purpose

    Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCm N-term MBP NikJC199U

    Plasmid

    #174364

    Purpose

    Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPPC016.109

    Plasmid

    #171144

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPPC008.106

    Plasmid

    #171141

    Purpose

    Expression of J106 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    J106 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-kcnj20

    Plasmid

    #173150

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj20 (si:ch211-113j13.2 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-kcnj12b

    Plasmid

    #173142

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj12b (kcnj12b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge