We narrowed to 4,045 results for: golden gate
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Plasmid#225821PurposeTn libraries Tn5 variant KAN backboneDepositorTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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pGLU_12
Plasmid#225820PurposeTn libraries Himar variant SPEC backboneDepositorTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_9
Plasmid#225817PurposeTn libraries Himar variant ERM backboneDepositorTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_11
Plasmid#225819PurposeTn libraries Himar variant CAM backboneDepositorTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-JIP60ml
Plasmid#104959PurposeEncodes a modified, active, form of Hordeum vulgare c.v. Golden Promise Jasmonate Induced Protein 60 (JIP60)DepositorInsertJasmonate Induced Protein 60 (JIP60)
UseGateway entry vector for lr recombination into ot…MutationReplaced amino acids 164 to 187 with a methionine…Available SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGI_Myr-YFP-MiniTurboID
Plasmid#222432PurposePotential plasma membrane control.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker, Myr, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKEK3233
Plasmid#234105PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
ExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO2
Plasmid#209976PurposeContains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (SH71_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPO1
Plasmid#209975PurposeContains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (256_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGTRm
Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…PromoterNoneAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASB
Plasmid#190175PurposePlasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequenceDepositorInsertsgRNA cloning site
ExpressionBacterial and YeastMutationAdded 5'-agagacc-3' upstream and 5'…Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKEK3230
Plasmid#234102PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor
Plasmid#128176PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralPromoterMSCVAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB2_AB
Plasmid#98534Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLP2
Plasmid#209989PurposeEntry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKEK3395
Plasmid#234079PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPO3
Plasmid#209977PurposeContains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pAMBeta1)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPR2
Plasmid#209980PurposeContains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmidsDepositorInsertresistance cassette (cat)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC54
Plasmid#209983PurposeContains Level 0* Part: 5' Connector (5C_07_5C1C) for the construction of Level 2 plasmidsDepositorInsert5' Connector (5C_07_5C1C)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC33
Plasmid#209984PurposeContains Level 0* Part: 3' Connector (3C_08_3C3) for the construction of Level 2 plasmidsDepositorInsert3' Connector (3C_08_3C3)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPR3
Plasmid#209986PurposeContains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmidsDepositorInsertresistance cassette (ermBL)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPO5
Plasmid#209987PurposeContains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmidsDepositorInsertreplication origin (256_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP3
Plasmid#209965PurposeContains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmidsDepositorInsertPromoter (P25)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP7
Plasmid#209969PurposeContains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_fabZ)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP8
Plasmid#209970PurposeContains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_gadB)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP9
Plasmid#209971PurposeContains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_ldhD)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPS2
Plasmid#209972PurposeContains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmidsDepositorInsertRBS (Lp)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPT2
Plasmid#209974PurposeContains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmidsDepositorInsertTerminator (T_pepN)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPR1
Plasmid#209979PurposeContains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmidsDepositorInsertresistance cassette (ermBL)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLP3
Plasmid#209958PurposeEntry vector to store Level 0 broad-host range L. plantarum replication origin partsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP1
Plasmid#209963PurposeContains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmidsDepositorInsertPromoter (mock)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP2
Plasmid#209964PurposeContains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmidsDepositorInsertPromoter (P48)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC31
Plasmid#209996PurposeContains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C2SN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC32
Plasmid#209997PurposeContains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C5CSN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC52
Plasmid#209991PurposeContains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C2SN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC51
Plasmid#209990PurposeContains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM098
Plasmid#216818PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold
TagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3ExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
BB2_EF
Plasmid#98538Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT100-neo
Plasmid#123137PurposeGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertNeomycin, poly-A terminator
UseSynthetic Biology; Golden gate donor vectorPromoternoneAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Multi-Janus-Spacer-1x_pKG1126
Plasmid#246375PurposeMulti-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
HSA_3b*_AP
Plasmid#238899PurposePlasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertRecombinant Human Serum Albumin (HSA) 3b*
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term_TEV_aMFx1 (4a)
Plasmid#238903PurposePlasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_TEV_aMFx1
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term_C-myc (4a)
Plasmid#238907PurposePlasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertC-term_C-myc
UseSynthetic BiologyAvailable SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H3
Plasmid#228189PurposePlasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P47001_CIS3_YEAST:0-21
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H4
Plasmid#228190PurposePlasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P80075_CCL8_HUMAN:0-23
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H5
Plasmid#228191PurposePlasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P31431_SDC4_HUMAN:0-20
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SP_H6
Plasmid#228192PurposePlasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).DepositorInsertsp_P59827_BPIB4_HUMAN:0-17
UseSynthetic BiologyAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only