Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,726 results for: golden gate

Clear keywords
Showing: 1701 - 1710 of 4726 results

  1. pAIP4_2ESK

    Plasmid
    #234232
    Purpose
    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2ESK (UBE2D2 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234232-plasmid-map-sequence-id-464196

    Enlarge

  3. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

    Enlarge

  4. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Christopher Bahl
    Insert
    Smt3 (SMT3 )
    Tags
    HisTag
    Promoter
    T7
    Available Since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233686-plasmid-map-sequence-id-468017

    Enlarge

  6. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

    Enlarge

  8. pUC-GW-Kan-L0-TaSEP1B6(int)

    Plasmid
    #242006
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum SEPALLATA1-6 (SEP1-6) B-subgenome homoeolog (TraesCS5B02G396700)
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242006-plasmid-map-sequence-id-504502

    Enlarge

  9. pDest_PANCEd_AraC_R2_S170_LOV_P1.1_C3

    Plasmid
    #242154
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P1.1.3 variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV P1.1.3
    Expression
    Bacterial
    Mutation
    AraC: E3K, R146L, A152T, R251L, E281K; AsLOV2: D5…
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242154-plasmid-map-sequence-id-492357

    Enlarge

  10. pDest_PANCEd_AraC_R2_S170_LOV_P2.1_C3

    Plasmid
    #242155
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P2.1.3 variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV P2.1.3
    Expression
    Bacterial
    Mutation
    AraC: E3K, E144K, R146L, A152T, S225R, R251L; AsL…
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242155-plasmid-map-sequence-id-492368

    Enlarge

Showing: 1701 - 1710 of 4726 results