We narrowed to 11,158 results for: HAL

1. pCMV-dCas9-mD3A

   Plasmid

   #78257

   Purpose

   Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

   Depositor

   Grant Challen

   Article

   McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

   Insert

   DNMT3A CD aa 598-912 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   E756A

   Promoter

   pCMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_Nsp3a_Ub1

   Plasmid

   #156467

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-Nix-MaoB

   Plasmid

   #100757

   Purpose

   Mammalian expression of mitochondria targetted Nix

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   Nix-MaoB (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TM domain replaced by MaoB

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_Orf10

   Plasmid

   #156486

   Purpose

   Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121842

   Purpose

   pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP

   Plasmid

   #166019

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Myc-Nix-CytoB5

   Plasmid

   #100756

   Purpose

   Mammalian expression of ER targeted Nix

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   Nix-CytoB5 (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TM domain replaced by CytoB

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_Nsp3c_SUD-N

   Plasmid

   #156469

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_Nsp3c_SUD-M

   Plasmid

   #156470

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSAVED-CHAT-cbm_PCaspase

    Plasmid

    #214047

    Purpose

    Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    lacUV5 promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSAVED-CHAT_PCaspase-N-C-term

    Plasmid

    #214046

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 1-153, 154-666

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSAVED-CHAT-sm_PCaspase

    Plasmid

    #214048

    Purpose

    Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJS-BCD-Strep-SAVED-CHAT

    Plasmid

    #214030

    Purpose

    Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS-BCD-Strep-dSAVED-CHAT

    Plasmid

    #214031

    Purpose

    Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    H375A, C422A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJS-BCD-Strep-SAVED-CHAT-sm

    Plasmid

    #214033

    Purpose

    Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS-BCD-Strep-PCaspase

    Plasmid

    #214034

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid

    #141100

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

    Plasmid

    #166020

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Use

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE30-VRN1-R249E-R289E-R296E

    Plasmid

    #159532

    Purpose

    Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

    Depositor

    Joshua Mylne

    Article

    King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

    Insert

    VERNALIZATION1 (VRN1 Mustard Weed)
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Three charge reversal mutations, namely R249E R28…

    Promoter

    T5

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ir7f-T2A-QF2 HDR plasmid

    Plasmid

    #140942

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    4 point mutations (annotated on plasmid map) were…

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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