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PE_26: Para_Lac_Tet_eG::sfYFP

Plasmid

#104887

Purpose

PE_26: Para_Lac_Tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Para_Lac_Tet_eG

Expression

Bacterial

Promoter

Para_Lac_Tet_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBALU-ext

Plasmid

#69339

Purpose

It allows to extend the genomic insert in a fosmid.

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

kanamycin gene +pCC1FOS homology

Expression

Bacterial

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pISH-mV2R55

Plasmid

#15866

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

mV2R55

Available Since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pPD177.62

Plasmid

#1701

Depositor

Andrew Fire

Type

Empty backbone

Tags

pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pPD172.01

Plasmid

#1719

Depositor

Andrew Fire

Type

Empty backbone

Tags

sur5-10k, sur5tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-iCRE-2A-H2BGFP-WPRE

Plasmid

#234893

Purpose

CAG-driven Cre recombinase coupled with H2B-GFP for nuclear fluorescent labelling

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

iCRE-2A-H2BGFP

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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HyWnt3:Luc

Plasmid

#192986

Purpose

Plasmid to test the Hydra Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCG150_double_phiC31_attB

Plasmid

#169693

Purpose

cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

Depositor

Shih-Peng Chan

Article

Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

Type

Empty backbone

Expression

Worm

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBA52_Ubiquitin

Plasmid

#110043

Purpose

Protein expression and purification of UBA52_Ubiquitin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBA52_Ubiquitin (UBA52 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-pat

Plasmid

#155028

Purpose

Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Phosphinothricin N-acetyltransferase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-bsr

Plasmid

#155027

Purpose

Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Blasticidin-S deaminase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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VAMP8-SuperNova

Plasmid

#131855

Purpose

Expresses SuperNova fused to endosomal SNARE VAMP8

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

Myc-tag and VAMP8 (mouse sequence)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-sbtA

Plasmid

#133011

Purpose

pFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

SbtA

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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EZ-L498

Plasmid

#131771

Purpose

PPGK1_FUSN_Citrine _PixE_TCYC1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PPGK1_FUSN_Citrine _PixE_TCYC1

Use

Integration into his3 locus

Expression

Yeast

Promoter

PGK

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSH86

Plasmid

#127706

Purpose

Pmyo-3::GFP1-10

Depositor

David M. Miller

Article

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

myo-3

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSH79

Plasmid

#127704

Purpose

Pttr-39::gfp1-10

Depositor

David M. Miller

Article

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

ttr-39

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FaGH17A

Plasmid

#86462

Purpose

Expression of the Beta-1,3-Endo-Laminarinase FaGH17A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH17A

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPD177.53

Plasmid

#1702

Depositor

Andrew Fire

Type

Empty backbone

Tags

pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc Affimer6-sfCherry2ΔN12

Plasmid

#231551

Purpose

Mammalian expression of actin-binding Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

sfCherry2

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-mApple-mito

Plasmid

#214415

Purpose

Mitochondria localization of low-affinity NFAST fused to mApple-mito

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Tags

mApple-mito

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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