We narrowed to 3,916 results for: LYC

1. pCAGGS D614G soluble Spike

   Plasmid

   #164651

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CAG

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFB1.HMBP.PrS.EED

   Plasmid

   #125163

   Purpose

   Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Polycomb protein EED (EED Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-UbC-eGFP

   Plasmid

   #62518

   Purpose

   Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

   Depositor

   Pantelis Tsoulfas, Jae Lee

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

   Plasmid

   #212538

   Purpose

   Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFB1.HMBP.PrS.SUZ12

   Plasmid

   #125162

   Purpose

   Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Polycomb protein SUZ12 (SUZ12 Human)
   

   Use

   Baculovirus expression vector

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFBOH-MHL.SUZ12

   Plasmid

   #125172

   Purpose

   Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LPUtopia-7

   Plasmid

   #199212

   Purpose

   Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Inserts

   aminoglycoside phosphotransferase,
   enhanced Green Fluorescence Protein
   Red Fluorescence Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and thymidine kinase promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 2XSmBiT-SARS-CoV-2 S1

   Plasmid

   #173430

   Purpose

   protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

   Depositor

   David Nemazee

   Article

   Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

   Insert

   2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
   

   Use

   Luciferase

   Tags

   human IgG1 Fc

   Expression

   Mammalian

   Mutation

   furin cleavage site RRAR to GSAS

   Promoter

   CMV

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-PGK-Chst3

    Plasmid

    #193024

    Purpose

    AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

    Insert

    Chst3 (Chst3 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid

    #70085

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

    Plasmid

    #170201

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV51

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-sACE2-T92Q-Fc(IgG1)

    Plasmid

    #145170

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mVenus-proα2(I)-G610C

    Plasmid

    #119839

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 SARS-CoV2 TEV-S

    Plasmid

    #154415

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of s…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA216-CoV2-RBD-beta variant

    Plasmid

    #194598

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

    Depositor

    Ben Murrell

    Article

    Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

    Insert

    CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCerulean-proα2(I)-G610C

    Plasmid

    #119837

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-SARS-CoV-2 S1 RBD

    Plasmid

    #167013

    Purpose

    Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    No stop codon

    Promoter

    Available since

    April 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pαH-S-GSAS-deltaH69/V70

    Plasmid

    #171744

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid

    #184408

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge