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pBa.GFP-AP1/gamma1

Plasmid

#218801

Purpose

Mammalian expression of mouse Ap1 g1

Depositor

Marvin Bentley

Article

Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

Insert

Ap1 g1 (Ap1g1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMIR-His-Halo-Tev-PTEN

Plasmid

#58241

Purpose

expresses halo tagged PTEN protein

Depositor

Yimon Aye

Article

Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

Insert

phosphatase and tensin homolog (PTEN Human)

Tags

His-Halo-Tev

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRN110

Plasmid

#127228

Purpose

CmYLCV::STM::35S terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

STM (STM A. tumefaciens, Mustard Weed)

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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CREB3L3

Plasmid

#99509

Purpose

to express CREB3L3 in mammalian system

Depositor

Dong-Yan Jin

Article

Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

Insert

CREB3L3 (Creb3l3 Mouse)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-FL

Plasmid

#120889

Purpose

Expresses wildtype Ptch1 for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-SI-121

Plasmid

#131126

Purpose

A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

mutant EGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc:P4:gs:nls:ABI

Plasmid

#135985

Purpose

Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the ABI domain

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-UAS-mIFNγ

Plasmid

#230004

Purpose

Gal4-dependent expression of secreted mouse IFNγ

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

UAS-mIFNγ (Ifng Synthetic)

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadDE

Plasmid

#193842

Purpose

Express TadDE (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadDE

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

Plasmid

#191145

Purpose

Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3UTR(Bdnf)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

Plasmid

#191141

Purpose

Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587-W:EGFP

Expression

Mammalian

Mutation

UAG>UGG (stop>Trp)

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GRAB_eCB2.0

Plasmid

#164608

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

CaMKII

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SB51

Plasmid

#48184

Purpose

Express full length HA tagged p35sr in mammalian cells

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCB6-Cor1-HA

Plasmid

#102815

Purpose

Expression of Coronin1 in eukaryotic cells

Depositor

Jean Pieters

Article

Gatfield et al Mol Biol Cell. 2005 Jun;16(6):2786-98. E

Insert

TACO/Coronin 1 (Coro1a Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Ub[9]-mRFP[1]

Plasmid

#20249

Depositor

Nico Dantuma

Article

Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

Insert

ubiquitin C (UBC Human)

Tags

mRFP

Expression

Mammalian

Mutation

changed Stop codon 686 to Glycine

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA HisB TO rFez1

Plasmid

#17385

Depositor

Stephen Goff

Article

Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Insert

FEZ1 (Fez1 Rat)

Tags

his and myc

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pJSK418

Plasmid

#205131

Purpose

Expression of GFP-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SI-112

Plasmid

#131127

Purpose

C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Mutant EGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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