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pRS416-Aga1p-FAPB2.3.6

Plasmid

#158147

Purpose

Yeast display reporter for non-display yeast strains (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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LD101

Plasmid

#69160

Purpose

Integration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg1-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAV0770

Plasmid

#133518

Purpose

Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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LD118

Plasmid

#69170

Purpose

Integration of GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg18-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFPQ4

Plasmid

#83559

Purpose

Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL7 promoter-yEGFP

Expression

Yeast

Promoter

GAL7

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAV0523

Plasmid

#133495

Purpose

Expression of nucleo-cytoplasmic sfGFP in M-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(mam1*)-sfGFP-terminator(ScADH1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDB1751

Plasmid

#174013

Purpose

pDUAL plasmid expressing GFP from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

GFP

Tags

GFP

Expression

Yeast

Promoter

41nmt1 and 41nmt1 promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pT-35

Plasmid

#16092

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

SMT3 (SMT3 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-TRP1-PGAL1-HBH

Plasmid

#26886

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pAV0771

Plasmid

#133513

Purpose

Expression of mTagBFP2 tagged CHD domain to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-3mTagBFP2-CHDRng2-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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LD220

Plasmid

#69188

Purpose

Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg20-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

Plasmid

#89071

Purpose

genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFS177

Plasmid

#12380

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

human equilibrating nucleoside transporter 1 (SLC29A1 Human)

Expression

Yeast

Mutation

N81S and M84V

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pUDP003

Plasmid

#101164

Purpose

E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAV0479

Plasmid

#133499

Purpose

Expression of sfGFP excluded from the nucleus with two NES signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0543

Plasmid

#133493

Purpose

Expression of nucleo-cytoplasmic mCherry in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pNB813

Plasmid

#60161

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pVD_85

Plasmid

#149604

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

XylR

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LD231

Plasmid

#69193

Purpose

Integration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg31-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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