We narrowed to 11,158 results for: HAL

1. pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

   Plasmid

   #222637

   Purpose

   Ezrin activation reporter, T567 phosphorylation deficient

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   T567A

   Promoter

   EF1a

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

   Plasmid

   #170202

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

   Plasmid

   #170203

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

   Plasmid

   #170191

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417N, E484K, N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVZ_PcoaT_SQE1_MRN1

   Plasmid

   #103858

   Purpose

   Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803

   Depositor

   Ilka Axmann

   Article

   Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

   Insert

   marneral synthase 1 (MRN1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   inserted genes are codon optimized2nd amino acid …

   Promoter

   PcoaT (from Synechocystis sp. PCC 6803)

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVZ_PcoaT_SQE1_CAS1

   Plasmid

   #103856

   Purpose

   Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803

   Depositor

   Ilka Axmann

   Article

   Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

   Insert

   cycloartenol synthase 1 (CAS1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   inserted genes are codon optimized. 2nd amino aci…

   Promoter

   PcoaT (from Synechocystis sp. PCC 6803)

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

   Plasmid

   #209077

   Purpose

   Ngn2 mediated cortical neuron induction, dox induction is included

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   NGN2 (Neurog2 Mouse)
   SNAPtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS426-HsFECH

   Plasmid

   #174524

   Purpose

   Expresses human ferrochelatase (FECH) in yeast

   Depositor

   Oleh Khalimonchuk

   Article

   Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

   Insert

   FECH with yeast HEM15 promoter and terminator (FECH Human, Budding Yeast, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Lacks original mitochondrial targeting sequence (…

   Promoter

   HEM15 (yeast, 257 bp)

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]

   Plasmid

   #108274

   Purpose

   AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   EF1α promoter (1.1kb short version)

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

    Plasmid

    #209083

    Purpose

    SNCA (Avi tagged) expression along with BirA ligase

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    BirA
    

    Use

    Tags

    Avi

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

    Plasmid

    #209084

    Purpose

    Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    hSyn

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid

    #209086

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid

    #209085

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EC71174

    Plasmid

    #154067

    Purpose

    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Tags

    Expression

    Bacterial and Plant

    Mutation

    The Arabidopsis thaliana U5 small nuclear ribonuc…

    Promoter

    35S promoter, Hordeum vulgare HSP 17 promoter use…

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPSW0CM0033

    Plasmid

    #197525

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Insert

    AtANR1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOZ0CM0220

    Plasmid

    #197527

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Insert

    AtIAA33
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPSW0CM0078

    Plasmid

    #197532

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Insert

    ANR1_w/o_stop
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF 1-SAG12-4

    Plasmid

    #20092

    Depositor

    Antonio Richart

    Insert

    SAG12
    

    Use

    Synthetic Biology; Plant gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPSW0CM0016

    Plasmid

    #197516

    Purpose

    Phytobricks Level 0 part

    Depositor

    Nicola Patron

    Insert

    AtANR1 (AT2G14210) 1000 bp upstream of TSS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-72B1

    Plasmid

    #59374

    Purpose

    Inducible expression of 71B2 (Q9M156) under T7 promoter

    Depositor

    Christopher Anderson

    Insert

    UGT72B1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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