We narrowed to 6,348 results for: Mag

1. pAAV9-hSyn-iCytSnFR_ER

   Plasmid

   #177752

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pAAV9-hSyn-iCytSnFR_ER
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV9-hSyn iCyt_BrEt_SnFR_ER

   Plasmid

   #177756

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pAAV9-hSyn iCyt_BrEt_SnFR_ER
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV9-hSyn-iCyt_F_SnFR_ER

   Plasmid

   #177754

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pAAV9-hSyn-iCyt_F_SnFR_ER
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

   Plasmid

   #175051

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Thr76 to Asp, phosphomimetic mutation

   Promoter

   T7 promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

   Plasmid

   #175050

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRepair-mTq2-CTNNB1

   Plasmid

   #153433

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   mTurquoise2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EcNGsC-DelFSH(307-309)-pKK223

   Plasmid

   #131377

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera DelFSH(307-309)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EcNGsC-S308P-H309D-pKK223

   Plasmid

   #131378

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308P H309D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EcNGsC-F307A-S308A-pKK223

   Plasmid

   #131379

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera F307A S308A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFUSEss-CHIg-mG3_VPEVS

    Plasmid

    #105863

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG3 heavy chain (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated heavy chain of mouse IgG3 with five mutat…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUSEss-CHIg-mG1_ILGGP

    Plasmid

    #105855

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1 heavy chain (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated heavy chain of mouse IgG1 with five mutat…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUSEss-CHIg-mG1_CH2charge

    Plasmid

    #105852

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutations modify charge of CH2 domain.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG1 heavy chain (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated heavy chain of mouse IgG1, muatations: Gl…

    Promoter

    hEF1-HTLV prom

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AL62_pDonor.DMD.TS

    Plasmid

    #100287

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Synthetic, Human)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBABE-puro p21 K161Q, K163Q

    Plasmid

    #78792

    Purpose

    Retroviral expression of p21 K161Q, K163Q

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    P21 (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K161Q, K163Q muation

    Promoter

    5'LTR

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCS3-MT-BX-6MYC p21 90-164

    Plasmid

    #78786

    Purpose

    To overexpress p21 90-164 in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    P21 (CDKN1A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    aa90-164

    Promoter

    CMV IE94

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCS3-MT-BX-6MYC p21 90-139

    Plasmid

    #78785

    Purpose

    To overexpress p21 90-139 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    contains p21 amino acids 89-139

    Promoter

    CMV IE94

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pBABE-puro p21 K161R, K163R

    Plasmid

    #78791

    Purpose

    Retroviral expression of p21 K161R, K163R

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K161R, K163R_muation

    Promoter

    5'LTR

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCardinal-Keratin-17

    Plasmid

    #56164

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

    Plasmid

    #158654

    Purpose

    making transgenics expressing a QUAS driven YFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Tags

    Expression

    Mutation

    F70A/F108Y

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-GCN5

    Plasmid

    #65386

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    GCN5 (KAT2A Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158653

    Purpose

    making transgenics expressing a UAS driven mCherry and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Tags

    Expression

    Mutation

    F70A/F108Y

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-APP-mGFP

    Plasmid

    #196696

    Purpose

    Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    HA and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-P3-Alb-mScarlet

    Plasmid

    #183461

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CBh-Alb-mNG

    Plasmid

    #183466

    Purpose

    To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge