We narrowed to 145,149 results for: ins

1. 2XMyc-LRRK2-WD40

   Plasmid

   #25073

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Contains only the WD40 domain aa 2155-2527

   Promoter

   Available since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pACE-GIRK4-S

   Plasmid

   #172425

   Purpose

   Expression of full-length, human GIRK4 with C-terminal StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMTH PKC zeta

   Plasmid

   #8414

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Mouse Protein Kinase C zeta (Prkcz Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pEAK-bKal7

   Plasmid

   #145800

   Purpose

   expresses Kalirin-7 with the b-front in mammalian cells

   Depositor

   Richard Mains, Betty Eipper

   Article

   Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

   Insert

   bKalirin-7 (Kalrn Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EIF2a

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFUGW-RapACR-EYFP

   Plasmid

   #111075

   Purpose

   Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 sf:mid.11

   Plasmid

   #191921

   Purpose

   Encodes a GFP design, sf:mid.11

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:mid.11
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Q69M Y145F T167I L220V V224I

   Promoter

   T7

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28 sf:mid.7

   Plasmid

   #191933

   Purpose

   Encodes a GFP design, sf:mid.7

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:mid.7
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72T Y145F V224I

   Promoter

   T7

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28 acid.6

   Plasmid

   #191912

   Purpose

   Encodes a GFP design, acid.6

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.6
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Q69L S72A Y145M T167V H181L

   Promoter

   T7

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28 sf:acid.3

    Plasmid

    #191926

    Purpose

    Encodes a GFP design, sf:acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:acid.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28 mid.7

    Plasmid

    #191900

    Purpose

    Encodes a GFP design, mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.7
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJRoC30_VP5H

    Plasmid

    #188061

    Purpose

    PROSS-designed versatile peroxidase 5H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Pleurotus ostreatus
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    43 PROSS mutations, described in publication

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-ZZ-TEV-SNAPf-BICD2N(1-400)

    Plasmid

    #111857

    Purpose

    N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICD2N(1-400)
    

    Use

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX SHIP2-SH2

    Plasmid

    #46493

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP2
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 8-131

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-PI(WT)-Src(KD)-mCherry

    Plasmid

    #87358

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    D388R

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNP1 tdTomato toehold switch

    Plasmid

    #107354

    Purpose

    Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 5 + tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2XMyc-LRRK2-COR

    Plasmid

    #25069

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the COR domain aa1408-1850

    Promoter

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pGen2.1-redCALWY-4

    Plasmid

    #50738

    Purpose

    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-4 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-ReaChR-citrine

    Plasmid

    #169045

    Purpose

    Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-citrine
    

    Use

    Gateway entry cloning vector

    Tags

    citrine

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MSP2279

    Plasmid

    #70706

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A and H557A in SaCas9

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBABE puro mouse IRS-2

    Plasmid

    #11371

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pGEX PLCg1(C)-SH2

    Plasmid

    #46469

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg1(C)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 656-766

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBABE bleo human IRS-1

    Plasmid

    #11448

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pGen2.1-redCALWY-1

    Plasmid

    #50739

    Purpose

    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-1 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX Syk(NC)-SH2

    Plasmid

    #46521

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(NC) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-263

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 3xFKBP-myc

    Plasmid

    #20213

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFKBP (FKBP1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pGEX-GP-2-MPHOSPH8 Chromo

    Plasmid

    #59694

    Purpose

    Contains histone modification interacting domain (MPHOSPH8 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    MPHOSPH8 Chromo (MPHOSPH8 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge