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Showing: 3481 - 3500 of 5008 results

  1. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid
    #183073
    Purpose
    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression
    Depositor
    Jennifer Doudna
    Article
    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4
    Insert
    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183073-plasmid-map-sequence-id-358157

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  3. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

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  4. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  5. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid
    #171588
    Purpose
    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
    Depositor
    Kenneth Matreyek
    Article
    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.
    Inserts
    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171588-plasmid-map-sequence-id-335078

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  6. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21916-plasmid-map-sequence-id-358974

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  7. pX333

    Plasmid
    #64073
    Purpose
    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    3XFLAG-Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CBh; U6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64073-plasmid-map-sequence-id-360030

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  8. pYPQ202

    Plasmid
    #86198
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis ubiquitin 10 (AtUbi10)
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86198-plasmid-map-sequence-id-174281

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  9. pmCherry-CIBN-CreC

    Plasmid
    #26889
    Purpose
    Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.
    Depositor
    Chandra Tucker
    Article
    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:
    Insert
    CIBN-CreC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    aa 1-170 of CIB1, aa 106-343 of Cre
    Promoter
    CMV
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    26889-plasmid-map-sequence-id-12741

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  11. pP2A_NLS_sfGFP

    Plasmid
    #63709
    Purpose
    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.
    Depositor
    Stanley Qi
    Article
    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.
    Insert
    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    Use
    Just a donor dna with nanog homologous arm for kn…
    Tags
    sfGFP
    Expression
    Mutation
    Promoter
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    63709-plasmid-map-sequence-id-107390

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  15. pRpr150-LUC

    Plasmid
    #85700
    Purpose
    luciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)
    Depositor
    Giannino Del Sal, Licio Collavin
    Article
    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-
    Insert
    RpR 150 enhancer and hsp70 minimal promoter
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    RpR 150 enhancer and hsp70 minimal promoter
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  19. pJK193

    Plasmid
    #71834
    Purpose
    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71834-plasmid-map-sequence-id-131053

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Showing: 3481 - 3500 of 5008 results