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We narrowed to 14,580 results for: pET

Showing: 3501 - 3520 of 14580 results

  1. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

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  7. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid
    #60866
    Purpose
    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    CohIII
    CBM
    Use
    Tags
    6xHIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    Cysteine 63 to Serine in CBM module
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60866-plasmid-map-sequence-id-98102

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  8. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  13. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid
    #203671
    Purpose
    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage
    Depositor
    Stephen Cusack
    Article
    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:
    Insert
    MCES_VACCA_D1_D12
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    203671-plasmid-map-sequence-id-403167

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  15. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid
    #159149
    Purpose
    expresses His-tagged HIV reverse transcriptase and untagged HIV protease
    Depositor
    Andrea Pauli
    Article
    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d
    Inserts
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    HIV-1 group M/subtype B – BH10 strain
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159149-plasmid-map-sequence-id-310074

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  17. pET-21a(+)-BMTU UDG-6xHis

    Plasmid
    #172197
    Purpose
    expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions
    Depositor
    Andrea Pauli
    Insert
    BMTU UDG
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    172197-plasmid-map-sequence-id-345439

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Showing: 3501 - 3520 of 14580 results