We narrowed to 4,353 results for: erf
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Plasmid#170753PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only
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p415:iCas9
Plasmid#138266PurposeExpression of mTN3-GGS6-dCas9 (iCas9) in yeast.DepositorInsertmTN3-GGS6-dCas9
UseCRISPRTagsExpressionYeastMutationCas9-D10A, H840APromoterGalLAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_030
Plasmid#123808PurposeEncodes NLS::dCAS9::NLS as a Type 3b part to be used in the MTK systemDepositorInsertNLS::dCAS9::NLS
UseTagsExpressionMammalianMutationPromoterAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.018
Plasmid#119622PurposeLevel 0 Part. CDSDepositorInsertdCas9+deg-tag
UseSynthetic BiologyTagsExpressionMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …PromoterAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-dCas9_O
Plasmid#127028PurposeBasic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.DepositorInsertPart dCas9_O
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24A
Plasmid#69462PurposeRNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A mutation
UseSynthetic BiologyTagsExpressionBacterialMutationC24A and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-A25C
Plasmid#69461PurposeRNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with A25C mutation
UseSynthetic BiologyTagsExpressionBacterialMutationA25C and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24AA25C
Plasmid#69463PurposeRNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A, A25C mutations
UseSynthetic BiologyTagsExpressionBacterialMutationC24A, A25C, and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-KRAB-MeCP2
Plasmid#122267PurposeSP-dCas9-KRAB-MeCP2 with doxycycline-inducible expressionDepositorInsertdCas9-KRAB-MeCP2
UseTagsdCas9-KRAB-MeCP2ExpressionMammalianMutationPromoterAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pdCRISPomyces-2BD
Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRTagsExpressionMutationPromoterAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-hfYFP
Plasmid#191201PurposeConstitutive CAG-driven expression of hfYFP in animals using AAVDepositorInsertHyperfolder YFP
UseAAVTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCAGAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[N150TAA]
Plasmid#164581Purposesuperfolder GFP with N150TAA mutationDepositorInsertsfGFP-150TAA
UseTags6xHisExpressionBacterialMutationN150TAA MutationPromoterT7Available SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_sfGFP-TurboID
Plasmid#240226PurposeGateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)DepositorInsertsfGFP-TurboID
UseGateway shuttle vectorTagsExpressionMutationPromoterAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-TurboID-blast
Plasmid#222599PurposeExpress fussed dCas9-TurboID for proximity labelingDepositorInsertdCas9-TurboID
UseLentiviralTagsExpressionMutationPromoterEF-1αAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationQ58LPromoterT5Available SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only