We narrowed to 17,342 results for: Ret;
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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiRhoA2G
Plasmid#40179DepositorInsertRhoA Biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-sfTq2ox
Plasmid#124220PurposePeriplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,DepositorInsertOmpA-mNG-sfTq2ox
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIC291
Plasmid#51403PurposeRetroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)DepositorInsertSka1 IMAGE 3909951 (SKA1 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATF6
Plasmid#32955PurposeFor visualization of GFP-ATF6 in mammalian cells.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationM67L and M313IPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Man1B1-GFP
Plasmid#163646PurposeExpresses GFP tagged Man1B1 in mammalian cellsDepositorInsertmannosidase alpha class 1B member 1 (MAN1B1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6-210
Plasmid#86919PurposeFluorescent reporter for ER calcium signalingDepositorInsertER-GCaMP6-210
UseLentiviralTagsExpressionMammalianMutationK78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…PromoterCAMKIIAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-PNGT3-d2EGFP_CMV-mCherry
Plasmid#213765PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertPNGT#3-d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNA motif plasmid cloning backbone
Plasmid#107253PurposeRNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kbDepositorInsertRNA motif plasmid cloning backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK_Gaussia_luciferase
Plasmid#154459PurposeExpression of gaussia luciferase and firefly luciferaseDepositorInsertGaussia luciferase
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMy-BirA-T2A-eGFP
Plasmid#50565PurposeRetroviral vector encoding for GFP and the biotinylating enzyme BirADepositorInsertAmpicilin
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE687
Plasmid#167299PurposeVector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAK11
Plasmid#227095PurposeLuciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vectorDepositorInsertCMV Promoter and HIV-1 TAR region
UseLuciferaseTagsExpressionMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
RINS1(mut)
Plasmid#107291PurposePlasmid for the expression of the inactive mutant of RINS1 sensorDepositorInsertRINS1(mut)
UseTagssfGFPExpressionMammalianMutationtwo Arg/Ser mutations at the beginning and in the…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-puro_G-CEPIA1er
Plasmid#164590PurposeStable integration and expression of G-CEPIA1er in mammalian cellsDepositorInsertG-CEPIA1er
UseLentiviralTagsMycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-EGFP
Plasmid#182641PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, EGFP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#73211Purposeexpress Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)DepositorInsertAAP2
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMW41: pET-His-MBP-SUMO-R2Bm-SII
Plasmid#209290PurposeExpresses the R2Bm ORF in E. coliDepositorInsertR2
UseTagsHis, MBP, StrepII, and bdSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK1520_puroR
Plasmid#173901PurposeSpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMV/U6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-Sec61 beta
Plasmid#49155Purposeexpression of Sec61 beta fused to mCherry, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsmCherry*ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2
Plasmid#17218PurposeRetroviral expression of human SOX2DepositorInsertSOX2 (SOX2 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Hygro_RGECO1.2
Plasmid#164592PurposeStable integration and expression of RGECO1.2 in mammalian cellsDepositorInsertR-GECO1.2
UseLentiviralTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125228PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
