We narrowed to 22,373 results for: Sis

1. Myc-CYLD-C601A

   Plasmid

   #60027

   Purpose

   Myc-tagged CYLD catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE80L-SH3

   Plasmid

   #160331

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   SH3
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKan-PCUP1-AID*(N)-9myc

   Plasmid

   #99526

   Purpose

   N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Expression

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/MSCV-EGFP

   Plasmid

   #82613

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

   Insert

   3xFLAG-dCas9
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-mCherry-SPASTIN

   Plasmid

   #180647

   Purpose

   Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, has siRNA resistance to GAACAGUGUG…

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSNV2 CHER NLS cop1at(335-675)

   Plasmid

   #44972

   Depositor

   Thanos Halazonetis

   Article

   Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

   Insert

   mCherry-NLS-COP1 (COP1 Mustard Weed)
   

   Use

   Tags

   NLS and mCherry

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-334

   Promoter

   pCMV IE

   Available since

   June 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMSCVneo

   Plasmid

   #134982

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgSlc17a7

   Plasmid

   #124846

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJY-RpABE-PDS3_IMS

   Plasmid

   #112875

   Purpose

   binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

   Depositor

   Jae-Young Yun, Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA targeting PDS3
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. NPY-mApple

    Plasmid

    #83498

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRosa26-TG

    Plasmid

    #40026

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (ATG)
    beta-globin intron
    Neo
    GFP
    diphteria toxin A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides 1-274 and insertion of FRT, …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC841 - pAAV minP secNLuc

    Plasmid

    #82498

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-alpha(740-977)-GFP

    Plasmid

    #100731

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2A1 (Ap2a1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-C1-FAK-HA-V744G

    Plasmid

    #35040

    Depositor

    Anna Huttenlocher

    Article

    Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

    Insert

    focal adhesion kinase (Ptk2 Mouse)
    

    Use

    Tags

    2x HA and mCherry

    Expression

    Mammalian

    Mutation

    GFP-FAK-V744G was generated from GFP-FAK using th…

    Promoter

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7C-DNAJB6

    Plasmid

    #177657

    Purpose

    Recombinant protein synthesis of human DNAJB6

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB6 (DNAJB6 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 AND EF1a

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid

    #84730

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Htr5b(3.7)-EGFP-sv40

    Plasmid

    #184411

    Purpose

    AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pS848∙CsR

    Plasmid

    #199240

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7C-DNAJB4

    Plasmid

    #177656

    Purpose

    Recombinant protein synthesis of human DNAJB4

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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