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pBbdCas9S_P0526-sgRNA500

Plasmid

#149641

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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(P5)pdc

Plasmid

#251385

Purpose

Gene encoding pyruvate decarboxylase (Zymomonas mobilis)

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

pdc

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GG

Plasmid

#213039

Purpose

AAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)

Depositor

Fredrik Sterky

Article

De La Cruz et al Bio Protoc. 2024 Nov 5;14(21):e5097. doi

Type

Empty backbone

Use

AAV; Vector for golden gate assembly

Tags

N/A

Promoter

N/A

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE3G_G

Plasmid

#240261

Purpose

Circuit-responsive (TRE3G promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during tTA/rtTA-3G campaigns.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

VSV-G

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_CBh

Plasmid

#241376

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with CBh promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH147

Plasmid

#186647

Purpose

aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPD301 ZF43x16-Compact_EYFP

Plasmid

#138911

Purpose

EYFP reporter vector for the ZF43x16-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x16-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x16-Compact

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD299 ZF43x14-Compact_EYFP

Plasmid

#138909

Purpose

EYFP reporter vector for the ZF43x14-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x14-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x14-Compact

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD294 ZF43x10-Compact_EYFP

Plasmid

#138905

Purpose

EYFP reporter vector for the ZF43x10-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x10-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x10-Compact

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2D'(sfGFP)

Plasmid

#126994

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1D'(sfGFP)

Plasmid

#126981

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1A(lacZ)

Plasmid

#126978

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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