We narrowed to 8,240 results for: FIE

1. pgRNA_hMECP2_promoter_No3

   Plasmid

   #194898

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pgRNA_hMECP2_promoter_No9

   Plasmid

   #194903

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDBEcR/Bla

   Plasmid

   #201556

   Purpose

   Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSUM-Kan-MCS2

   Plasmid

   #109289

   Purpose

   Expresses high levels of protein in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pSUM-kan-MCS1-gfp was modified to reorient the Ba…

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAT.000

   Plasmid

   #119559

   Purpose

   Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRC1765

   Plasmid

   #141346

   Purpose

   Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

   Depositor

   Rafael Fernandez-Leiro

   Article

   Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDF0039 DisCas7-11 only expression with CRISPR array

   Plasmid

   #172500

   Purpose

   Encodes the DisCas7-11 protein only for bacterial expression

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 only expression with CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

   Plasmid

   #129650

   Purpose

   Rieske FeS overexpression

   Depositor

   Susanne von Caemmerer

   Article

   Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

   Insert

   CDS of PetC gene encoding for the Rieske FeS subunit
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon modified for Golden Gate

   Promoter

   pZmUbi

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JC001: pMVP/Ad/RGD-DEST

   Plasmid

   #121844

   Purpose

   pMVP destination vector, empty RGD fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiCHECK3-SDHAF3

    Plasmid

    #136013

    Purpose

    SDHAF3 3'UTR (NM_020186.2) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    SDHAF3 3'UTR (ACN9 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C-BLa 2.0

    Plasmid

    #92060

    Purpose

    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Use

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTorPE-B-GECO1

    Plasmid

    #32469

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    B-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

    Plasmid

    #207665

    Purpose

    Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

    Depositor

    Robert de Vries

    Article

    Spruit et al bioRxiv 2023.12.15.571923

    Insert

    Fucosidase E1_10125
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    amino acids 34-584 were used

    Promoter

    T7 promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-EGFPnls barcode-206-SV40 polyA

    Plasmid

    #190875

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode206
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JH401: pMVP/Ad/RGD-PK-DEST

    Plasmid

    #121846

    Purpose

    pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAP118

    Plasmid

    #105495

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-EGFP barcode-10-SV40 polyA

    Plasmid

    #190874

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCellFree_G03 ISG15

    Plasmid

    #67054

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMTTH+eGFP-bromo5(N140A)

    Plasmid

    #157806

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5(N140A)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    N140A

    Promoter

    pTAC

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-Dusp6_2KBpromoter

    Plasmid

    #32666

    Depositor

    Michael Tsang

    Article

    Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

    Insert

    Dusp6 (dusp6 Zebrafish)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pgRNA_hMECP2_promoter_No4

    Plasmid

    #194899

    Purpose

    Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Insert

    sgRNA targeting human MECP2 promoter No.4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTK158_mCherry-4.1G-deltaCTD-hardened

    Plasmid

    #46360

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 886-1005, and modified for si…

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDGB1omega2_SF (GB0466)

    Plasmid

    #68243

    Purpose

    Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDGB1alpha2_SF (GB0107)

    Plasmid

    #68233

    Purpose

    Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET11-PC-H6

    Plasmid

    #183777

    Purpose

    Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.

    Depositor

    Peter Harte

    Article

    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

    Insert

    Polycomb (Pc Fly)
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1/SPTBN1-FLT3

    Plasmid

    #168115

    Purpose

    Mammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemia

    Depositor

    Nicholas Cross

    Article

    Grand et al Exp Hematol. 2007 Nov;35(11):1723-7. doi

    Insert

    SPTBN1-FLT3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcFLAG-Grap2-WT

    Plasmid

    #74511

    Purpose

    Overexpression of Grap2 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

    Insert

    Grap2 (GRAP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pONSY

    Plasmid

    #111873

    Purpose

    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Type

    Empty backbone

    Use

    Capsaspora owczarzaki

    Tags

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pR6Kg-Alpha1spm

    Plasmid

    #165866

    Purpose

    Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMpGWB117

    Plasmid

    #68571

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GB1-His-SAP25-126-186(pL91)

    Plasmid

    #124387

    Purpose

    E.Coli produced SAP25 SID purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-SAP25-126-186 (SAP25 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pXW109Hg(RS-RinA)

    Plasmid

    #123150

    Purpose

    Mercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMpGWB118

    Plasmid

    #68572

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMpGWB218

    Plasmid

    #68609

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Tags

    Expression

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTorPE-GEX-GECO1

    Plasmid

    #32464

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMpGWB217

    Plasmid

    #68608

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMpGWB320

    Plasmid

    #68648

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXW109ABS84(RS)

    Plasmid

    #123155

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS84, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSUM-GroEL-gfp

    Plasmid

    #109286

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GroEL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    GroEL

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge