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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,419 results for: BLI;

Showing: 3561 - 3580 of 8419 results

  1. pmSod1-D83G-N’His

    Plasmid
    #117702
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D83G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117702-plasmid-map-sequence-id-227517

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  2. pHH0103 PQBP1 WW domain

    Plasmid
    #104287
    Purpose
    Bacterial expression of WW domain from PQBP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PQBP1 WW domain (PQBP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104287-plasmid-map-sequence-id-203724

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  3. pHH0103 HLA-DQA2 WW domain

    Plasmid
    #104297
    Purpose
    Bacterial expression of WW domain from HLA-DQA2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HLA-DQA2 WW domain (HLA-DQA2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104297-plasmid-map-sequence-id-203688

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  4. TAP-Amotl2

    Plasmid
    #112834
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    TAP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112834-plasmid-map-sequence-id-217933

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  5. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104977-plasmid-map-sequence-id-202891

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  6. pHH0103 HYPC WW domain #2

    Plasmid
    #104232
    Purpose
    Bacterial expression of WW domain #2 from HYPC
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HYPC WW domain #2 (PRPF40B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104232-plasmid-map-sequence-id-204196

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  7. GLT-1b delta 4

    Plasmid
    #98707
    Purpose
    mammalian expression of Glt1b delta 4 mutant
    Depositor
    Paul Rosenberg
    Insert
    Glt1b (Slc1a2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    missing last 4 amino acids at the C-terminus. I52…
    Promoter
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    98707-plasmid-map-sequence-id-193958

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  8. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  9. pCASCADE-udhA-zwf

    Plasmid
    #87153
    Purpose
    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA-zwf guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87153-plasmid-map-sequence-id-174933

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  10. pGEM-floxedEGFP-TRI2A

    Plasmid
    #74669
    Purpose
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Depositor
    Roberto Giovannoni
    Insert
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    pCAGGS
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    74669-plasmid-map-sequence-id-147558

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  11. pJK545

    Plasmid
    #74225
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains a silent mutation in Ala63 codon
    Promoter
    T7
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74225-plasmid-map-sequence-id-142021

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  12. pJK139

    Plasmid
    #73315
    Purpose
    Pas2p peroxisomal membrane biogenesis protein
    Depositor
    T. J. Kappock
    Insert
    Pas2p peroxisomal membrane targeting protein
    Use
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    Encodes a glycine-204 to serine mutation
    Promoter
    AOX1
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73315-plasmid-map-sequence-id-140431

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  13. pJK267

    Plasmid
    #72432
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Insert
    Acetobacter aceti 1023 gDNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    n/a
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pJK137

    Plasmid
    #73313
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    5'-AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73313-plasmid-map-sequence-id-135010

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  15. pJK361

    Plasmid
    #72403
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72403-plasmid-map-sequence-id-134630

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  16. pJK108

    Plasmid
    #73312
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73312-plasmid-map-sequence-id-135009

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  17. pJK368

    Plasmid
    #72407
    Purpose
    Produces Acetobacter aceti 1023 Est12
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72407-plasmid-map-sequence-id-135653

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  18. pJK692

    Plasmid
    #71643
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan, serine-38 to …
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71643-plasmid-map-sequence-id-131664

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  19. pJK653

    Plasmid
    #71630
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine, phenylalanine-79 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71630-plasmid-map-sequence-id-131649

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  20. pJK654

    Plasmid
    #71631
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine, phenylalanine-79 to…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71631-plasmid-map-sequence-id-131651

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Showing: 3561 - 3580 of 8419 results