We narrowed to 24,603 results for: Nes

1. pMaCTag-P34

   Plasmid

   #120030

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xFLAG
   

   Use

   Pcr template

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. phCMV-IE1

   Plasmid

   #118048

   Purpose

   GBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Cytomegalovirus enhancer and promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P28

   Plasmid

   #120039

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xHA
   

   Use

   Pcr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEC2861 (pSpy1C-PgyrA_ffluc)

   Plasmid

   #218511

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PgyrA

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-Z29

   Plasmid

   #120072

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Omega Destination-CMV::ELuc:bGH

   Plasmid

   #124528

   Purpose

   Omega destination vector, that has a CMV:ELUC:bgH in the backbone

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Type

   Empty backbone

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 35S::GeNL/pCAMBIA1301

   Plasmid

   #190070

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Green-enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTD103-ARG1

   Plasmid

   #106475

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRANS_253

   Plasmid

   #91125

   Purpose

   Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-Z05

    Plasmid

    #120048

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMC-1-WDV-LRS-L-11

    Plasmid

    #197720

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    WDV LRSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRoLuc

    Plasmid

    #135284

    Purpose

    GBPart - RoLuc Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    R. ohbai Luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. c3GIC9

    Plasmid

    #62191

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-HpaBC

    Plasmid

    #71260

    Purpose

    Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

    Inserts

    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAN-PTet-dCas9

    Plasmid

    #62244

    Purpose

    controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    catalytically inactive, D10A, H840A

    Promoter

    Ptet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRANS_211

    Plasmid

    #91110

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nano-lantern/pRSETB

    Plasmid

    #51969

    Purpose

    bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. (R42A) Cyclin B1-GFP (62)

    Plasmid

    #61849

    Purpose

    mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

    Depositor

    Jonathon Pines

    Article

    Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    V5A R42A

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKM126

    Plasmid

    #105134

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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