We narrowed to 5,124 results for: PEP

1. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K both

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LifeAct-MTED

   Plasmid

   #217498

   Purpose

   Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

   Depositor

   Erik Dent

   Article

   Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

   Insert

   LifeAct-mScarlet-MTED
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVasa-Cre

   Plasmid

   #15885

   Depositor

   Diego Castrillon

   Article

   Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

   Insert

   mouse vasa promoter (Ddx4 Mouse)
   

   Use

   Tags

   Cre

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. mVenus-hCOL1A1

   Plasmid

   #140111

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-fNPY-GFP

   Plasmid

   #22912

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   fugu neuropeptide Y promoter driving GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. mApple-hCOL1A1

   Plasmid

   #140113

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mNFAT4-462:TALE:VP64:KRɸ

   Plasmid

   #160662

   Purpose

   Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   mNFAT4-462:TALE:VP64:KRɸ
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid

    #160688

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA Polymerase II CTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C terminal domain only

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST14-Avitag-SpyCatcher002

    Plasmid

    #102828

    Purpose

    Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Avitag-SpyCatcher002
    

    Use

    Tags

    Avitag, GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCMV_PACLight1

    Plasmid

    #197864

    Purpose

    Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

    Depositor

    Tommaso Patriarchi

    Article

    Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

    Insert

    PACLight1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Promoter

    Available since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNK869

    Plasmid

    #219737

    Purpose

    MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

    Insert

    promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtPD7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCerulean-hCOL1A1

    Plasmid

    #140112

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-GFP-Dub3 mouse

    Plasmid

    #72683

    Purpose

    mammalian expression of GFP-Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB-LIC-Bse

    Plasmid

    #26108

    Purpose

    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His6 - TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Tras light chain

    Plasmid

    #216294

    Purpose

    Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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