We narrowed to 1,215 results for: calcium

1. pQE-9-RTXMCR-TLY-X2E-C

   Plasmid

   #225976

   Purpose

   Repeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXMCR-TLY-X2E-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE-9-RTXWTM-X2A-C

   Plasmid

   #225965

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of alanine in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2A-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE-9-RTXWTM-X2H-C

   Plasmid

   #225966

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with global substitution of histidine in nonapeptide repeats and C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWTM-X2H-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-LgBiT-GAI

   Plasmid

   #183405

   Purpose

   Expresses nuclear-localized LgBiT-GAI

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-GAI
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-D-TOPO-kcnt2b

   Plasmid

   #164964

   Purpose

   Zebrafish kcnt2a gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnt2b (LOC565691 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-kcnt1a

   Plasmid

   #164961

   Purpose

   Zebrafish kcnt1a gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnt1a (kcnt1 Zebrafish)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. cp173-mCer3-C1

   Plasmid

   #105752

   Purpose

   a circularly permuted mCerulean3 for FRET sensor optimization

   Depositor

   Megan Rizzo

   Article

   Markwardt et al J Biol Chem. 2016 Feb 5;291(6):3000-9. d

   Insert

   cp173-mCer3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PCx34.7IL-pET42

   Plasmid

   #42915

   Depositor

   John O'Brien

   Article

   Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

   Insert

   Connexin 34.7 cytoplasmic loop
   

   Use

   Tags

   6x His, GST, and S-tag

   Expression

   Bacterial

   Mutation

   cytoplasmic loop is aa 102-179

   Promoter

   T7

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-SomaGCaMP7

   Plasmid

   #158759

   Purpose

   Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

   Depositor

   Edward Boyden

   Article

   Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

   Insert

   SomaGCaMP7
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-SomaGCaMP6f2

    Plasmid

    #158756

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP6f2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-SomaGCaMP6f2

    Plasmid

    #158757

    Purpose

    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Has Service

    AAV8 and AAV9

    Insert

    SomaGCaMP6f2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-SomaGCaMP6f1

    Plasmid

    #158755

    Purpose

    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP6f1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid

    #158758

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    nullCoChR-GCaMP6f-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TRE-mCherry

    Plasmid

    #92202

    Purpose

    Fluorescent protein reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDest-mCherry-N1

    Plasmid

    #31907

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 10TALE_Pmin_fLuc

    Plasmid

    #160663

    Purpose

    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    10TALE_Pmin_fLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ER-GCaMP6f

    Plasmid

    #182548

    Purpose

    Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-M13-TEV-C-P2A-TdTomato

    Plasmid

    #92389

    Purpose

    expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    M13-TEV-C-P2A-TdTomato
    

    Use

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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