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PHAGE-P CMVt N-HA hE6-AP I (R39H)

Plasmid

#119309

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (R39H) (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

G20V

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

Plasmid

#119314

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Only AA 55-852

Promoter

CMV

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-HA-UBE3BΔHECT-IRES-Puro

Plasmid

#107347

Purpose

Expresses HA tagged UBE3B deletion of HECT domain in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3BΔHECT (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

deletion HECT domain (757-end)

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)

Plasmid

#119315

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Only AA 366-852

Promoter

CMV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

Plasmid

#119316

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Only AA 481-852

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

Plasmid

#119318

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

C41A, C46A, C840A

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0926

Plasmid

#143240

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

Plasmid

#202761

Purpose

Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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