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We narrowed to 434 results for: T7 rna polymerase

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Showing: 341 - 360 of 434 results

  3. pRN3P_T3_BE3_IVT

    Plasmid
    #177015
    Purpose
    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase
    Depositor
    Timo Otonkoski
    Publication
    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    177015-plasmid-map-sequence-id-348280

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  4. pRN3P_T3_BE4_IVT

    Plasmid
    #171762
    Purpose
    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase
    Depositor
    Timo Otonkoski
    Publication
    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE4
    Use
    Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    171762-plasmid-map-sequence-id-346879

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  8. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Publication
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  15. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  16. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99214-plasmid-map-sequence-id-192369

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  17. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  20. AV15_pCAG.Cas9.gRNA.S1

    Plasmid
    #199259
    Purpose
    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199259-plasmid-map-sequence-id-391662

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Showing: 341 - 360 of 434 results