We narrowed to 8,240 results for: FIE

1. pSum-LacZ-gfp

   Plasmid

   #109285

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   LacZalpha

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

   Plasmid

   #129539

   Purpose

   modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

   Depositor

   Arneet Saltzman

   Article

   Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox; Mos1-mediated single copy insertion (mos…

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-ChroME(F243Y)-mRuby2-ST

   Plasmid

   #109127

   Purpose

   Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChroME(F243Y)-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-CMV-p85a 3'UTR mutant

   Plasmid

   #51393

   Depositor

   Narry Kim

   Article

   Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

   Insert

   p85alpha 3'UTR (PIK3R1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   miR-29 binding site mutant

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

5. p230-pOsAct1::hpt-pZmUbi::BdPetC

   Plasmid

   #129649

   Purpose

   Rieske FeS overexpression

   Depositor

   Susanne von Caemmerer

   Article

   Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

   Insert

   CDS of PetC gene encoding for the Rieske FeS subunit
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon modified for Golden Gate

   Promoter

   pZmUbi

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP122

   Plasmid

   #21669

   Depositor

   James Haber

   Article

   Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

   Insert

   Lacz (lacZ E. coli)
   

   Use

   Centromeric plasmid

   Tags

   Expression

   Mutation

   The second lacZ sequence has been modified, the E…

   Promoter

   Available since

   Sept. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pYG026

   Plasmid

   #65328

   Purpose

   reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   ccdB
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMpGWB417

   Plasmid

   #68682

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMpGWB317

   Plasmid

   #68645

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGWB220

    Plasmid

    #68611

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFRT-TODestFLAGHA_RBPMS2_HindIII

    Plasmid

    #65666

    Purpose

    FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFP121

    Plasmid

    #21670

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

13. Syn3D38

    Plasmid

    #13397

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3D38 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. First N-…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pXJ-Myc-IRF2

    Plasmid

    #232642

    Purpose

    transient expresses IRF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF2 (IRF2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXJ-Myc-GATA3

    Plasmid

    #232646

    Purpose

    transient expresses GATA3 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    GATA3 (GATA3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXJ-Myc-KLF16

    Plasmid

    #232655

    Purpose

    transient expresses KLF16 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    KLF16 (KLF16 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Use

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-Dyskerin

    Plasmid

    #207577

    Purpose

    N-terminal mCherry-tag using SalI/EcoRI

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SOX D221A/E244A/K246A pACEmam1

    Plasmid

    #192050

    Purpose

    Expresses a 3X catalytic mutant of SOX (ORF37)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (3X catalytic mutant)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    3X catalytic mutant D221A/E244A/K246A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACE SOX (ORF37) K246A

    Plasmid

    #192051

    Purpose

    Expresses a catalytic mutant of SOX (K246A)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (K246A)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Catalytic mutant K246A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACE SOX (ORF37) K250A

    Plasmid

    #192052

    Purpose

    Expresses a DNA binding mutant K250A (ORF37)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (K250A)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    DNA binding mutant K250A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACE SOX (ORF37) 318-320A

    Plasmid

    #192054

    Purpose

    Expresses a mutant of SOX 318-320A

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (318-320A)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    SOX 318-320A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSF_ApyA

    Plasmid

    #226249

    Purpose

    Production of unmodified ApyA peptide.

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Insert

    His6_ApyA
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225062

    Purpose

    Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

    Plasmid

    #225061

    Purpose

    Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225063

    Purpose

    Expression of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTE5423_p3WJdB-glcO60

    Plasmid

    #221194

    Purpose

    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    T7 promoter-glcO60-3WJdB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter with downstream glcO60 operator

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L2383-FRB-GPA-NLuc

    Plasmid

    #222902

    Purpose

    Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEM-601[insert(+1) TA-rich]

    Plasmid

    #221196

    Purpose

    601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    601[insert(+1)TA-rich]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    601 has an additional mutation added 22 bp from d…

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 95.a_pSTC0-zeo

    Plasmid

    #202334

    Purpose

    Design 95.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    95.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 95.b_pSTC0-zeo

    Plasmid

    #202335

    Purpose

    Design 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    95.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 90.a_pSTC0-zeo

    Plasmid

    #202336

    Purpose

    Design 90.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    90.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 90.b_pSTC0-zeo

    Plasmid

    #202337

    Purpose

    Design 90.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    90.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 80.a_pSTC0-zeo

    Plasmid

    #202338

    Purpose

    Design 80.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    80.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 80.b_pSTC0-zeo

    Plasmid

    #202339

    Purpose

    Design 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    80.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 70.a_pSTC0-zeo

    Plasmid

    #202340

    Purpose

    Design 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    70.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 70.b_pSTC0-zeo

    Plasmid

    #202341

    Purpose

    Design 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    70.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 50.a_pSTC0-zeo

    Plasmid

    #202342

    Purpose

    Design 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    50.a
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 50.b_pSTC0-zeo

    Plasmid

    #202343

    Purpose

    Design 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    50.b
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. rw-consensus_pSTC0-zeo

    Plasmid

    #202349

    Purpose

    Control rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    rw-consensus
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge